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Inhibition and stimulation of respiration-linked Mg2+ efflux in rat heart mitochondria

Journal of Bioenergetics and Biomembranes volume 13, pages 133–139 (1981)Cite this article

Abstract

Respiration-driven Mg2+ efflux from rat heart mitochondria has been studied in different conditions. Almost total release of Mg2+ from the mitochondria occurs upon addition of a proton/bivalent cation exchanger, A23187. The content of Mg2+ remaining in mitochondria after A23187 treatment is the same if part of the mitochondrial Mg2+ has already been extruded through the energy-linked mechanism. Some inhibition of Mg2+ efflux is observed in the presence of high concentrations of La3+ (100 µM). A proton/monovalent cation exchanger, nigericin, completely prevents Mg2+ efflux, whereas a cation conductor, valinomycin, considerably stimulates it. The results indicate that the main part of mitochondrial Mg2+ is present in a membrane-bounded compartment, probably in the matrix space. The driving force of the Mg2+ efflux appears to be the proton gradient (ΔpH) created by mitochondrial respiration.

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Affiliations

  1. Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10 A, SF-00170, Helsinki 17, Finland

    Karl E. O. Åkerman

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  1. Karl E. O. Åkerman
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Åkerman, K.E.O. Inhibition and stimulation of respiration-linked Mg2+ efflux in rat heart mitochondria. J Bioenerg Biomembr 13, 133–139 (1981). https://doi.org/10.1007/BF00763835

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  • DOI: https://doi.org/10.1007/BF00763835

Key Words

  • Mg2+ efflux
  • heart mitochondria
  • respiration