Abstract
Adriamycin, 10−8–10−5 M, inhibited transplasma membrane electron transport of uninduced HL-60 cells susceptible to adriamycin and not in uninduced HL-60 cells resistant to adriamycin as measured by reduction of external ascorbate free radical. Electron flow across the plasma membrane was measured with the intact living cells by means of a simple assay procedure whereby the transported electrons were captured by ascorbate free radical to slow the rate of chemical oxidation of ascorbate. The response to adriamycin was rapid with maximum inhibition in less than 1 min. Preincubation was not required and the inhibition presumably was not mediated through effects on DNA replication or transcription. Except at the highest concentration tested of 10 µM, both transplasma membrane electron transport and growth were unaffected by adriamycin with a line of HeLa cells resistant to the drug. The findings provide evidence, using a physiological acceptor, ascorbate free radical, for a direct inhibition of transmembrane electron transport of HL-60 cells by adriamycin that correlates closely with adriamycin inhibition of cell growth. The lack of response with resistant cells suggests an alternative mechanism for adriamycin resistance not necessarily based on transport control.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alcain, F. J., Burón, M. I., Villalba, J. M., and Navas, P. (1991).Biochem. Biophys. Acta 1073 380–385.
Algeria, A. E., Rodriguez, M. S., and Hernandez, J. (1990).Biochim. Biophys. Acta 1035 51–55.
Arcamone, F. (1985).Cancer Res. 45 5995–5999.
Bachur, H. R., Gordon, S. L., Gee, M. V., and Kon, H. (1979)Proc. Natl. Acad. Sci. 76 945–957.
Barabas, K., Sizensky, J. A. and Faulk, W. P. (1991).Am. J. Reprod. Immunol. 25 120–124.
Burke, T. G., and Tritton, T. R. (1985).Biochemistry 24 5972–5980.
Clark, M. G., Patrick, E. J., Patten, G. S., Crane, F. L., Löw, H., and Grebing, C. (1981).Biochem. J. 200 565–572.
Crane, F. L., Crane, H. E., Sun, I. L., MacKellar, W. C., Grebing, C., and Löw, H. (1980).Biochem. Biophys. Res. Commun. 93 746–754.
Crane, F. L., Sun, I. L., Clark, M. G., Grebing, C., and Löw, H. (1985).Biochim. Biophys. Acta 811 233–264.
Crane, F. L., Löw, H., and Morré, D. J. (1990). InOxidoreduction at the Plasma Membrane: Relation to Growth and Transport (Crane, F. L., Morré D. J., and Löw, H., eds.), C.R.C. Press, Boca Raton, Florida. Vol. 1, pp. 1–27.
Cullianane, C., and Phillips, D. R. (1990).Biochemistry 29 5638–5645.
Faulk, W. P., Hsi, B. L., and Stevens, P. J. (1980).Lancet 2 390–392.
Faulk, W. P., Torry, D. S., Harats, H., McIntyre, J. A., and Taylor, C. G. (1988). InPlasma Membrane Oxidoreductases in Control of Animal and Plant Growth (Crane, F. L., Morré, D. J., and Löw, H. eds.), Plenum, New York, pp. 173–180.
Faulk, W. P., Taylor, C. G., Yeh, C. J. G., and McIntyre, J. A. (1990a).Mol. Biotherm. 2 57–60.
Faulk, W. P., Harats, H., and Berczi, A. (1990b). InOxidoreduction at the Plasma Membrane: Relation to Growth and Transport Crane, F. L., Morré D. J., and Löw, H., eds.), CRC Press, Boca Raton, pp. 205–224.
Morré, D. J., Crane, F. L., Eriksson, L. C., Löw, H., and Morré, D. M. (1991).Biochim. Biophys. Acta 1057 140–146.
Navas, P., Alcain, F., Burón, M. I., Rodriquez-Aguilera, J. C., Villalba, J. M., Morré, D. M., and Morré, D. J. (1992).FEBS Lett. 299 223–226.
Patrick, D. (1988).Cancer Invest. 6 439–451.
Powis, G. (1989).Free Radical Biol. Med. 6 63–101.
Rogers, K. E., and Tokes, Z. A. (1984).Biochem. Pharmacol. 33 605–608.
Solie, T. N., and Yuncker, C. (1978).Life Sci. 22 1907–1920.
Sun, I. L., and Crane, F. L. (1984).Proc. Indiana Acad. Sci. 93 267–274.
Sun, I. L., and Crane, F. L. (1988). InPlasma Membrane Oxidoreductases in Control of Animal and Plant Growth (Crane, F. L., Morré, D. J., and Löw, H., eds.), Plenum Press, New York, pp. 181–190.
Sun, I. L., and Crane, F. L. (1990). InOxidoreduction at the Plasma Membrane: Relations to Growth and Transport, Vol. I, Animals (Crane, F. L., Morré, D. J., and Löw, H., eds.), CRC Press, Boca Raton, pp. 257–280.
Sun, I. L., Crane, F. L., Chou, J. Y., Löw, H., and Grebing, C. (1983).Biochem. Biophys. Res. Commun. 116 649–654.
Sun, I. L., Crane, F. L., and Chou, J. Y. (1986).Biochim. Biophys. Acta 886 327–336.
Sun, I. L., Navas, P., Crane, F. L., Morré, D. J., and Löw, H. (1987).Biochem. Int. 14 119–127.
Sun, I. L., Sun, E. E., Crane, F. L., Morré, D. J., Lindgren, A., and Löw, H. (1992)Proc. Natl. Acad. Sci. 89 11126–11130.
Tewey, K. M., Rowe, T. C., Yand, I., Halligan, B. D., and Liu, L. F. (1984).Science 266 466–468.
Thorstensen, K., and Romslø, I. (1990).Biochem. J. 271 1–10.
Tokes, Z. A., Rogers, K. E., and Rembaum, A. (1982).Proc. Natl. Acad. Sci. 79 2026–2030.
Tritton, T. R., and Yee, G. (1982).Science 217 248–250.
Vichi, P., and Tritton, T. R. (1992).Cancer Res. 52 4135–4138.
Winkler, B. S. (1987).Biochim. Biophys. Acta 925 258–264.
Yeh, C. J., and Faulk, W. P. (1984).Clin. Immunol. Immunopathol. 32 1–11.
Yeh, C. J. G., Taylor, C. G., and Faulk, W. P. (1984).Proteids Biol. Fluids 32 441–444.
Yokayama, M., Miyauchi, M., Yamada, N., Okano, T., Sakarai, Y., Kataoka, K., and Inoue, S. (1990).Cancer Res. 50 1963–1970.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morré, D.J., Morré, D.M. & Wu, LY. Response to adriamycin of transplasma membrane electron transport in adriamycin-resistant and nonresistant HL-60 cells. J Bioenerg Biomembr 26, 137–142 (1994). https://doi.org/10.1007/BF00763225
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00763225