Abstract
Many effects of ascorbate center on its interactions with membranes from plant and animal cells. These actions can be studied using vesicles produced from phospholipid components (liposomes), by isolating naturally occurring vesicles, or by purifying particular membranes that form vesicles during the extraction process. Liposomes have provided information concerning the anti- and prooxidant properties of ascorbate and about how the water-soluble vitamin can have effects within the phospholipid bilayer. The involvement of ascorbate in transmembrane electron transport has been characterized in vesicles normally found in certain cells, such as, chromaffin granules, synaptosomes, glyoxisomes, peroxisomes, and clathrincoated vesicles. Redox activity using reducing power associated with ascorbate/ascorbate free radical (AFR) has been characterized in some of these vesicles and it appears to be mediated by ab-type cytochrome. Ascorbate also participates in the reduction of iron within clathrin-coated vesicles. Vesicles appearing during purification of plasma membranes have transmembrane electron transport, oxidoreductase activity with ascorbate/AFR as redox agents, and an ascorbate-reducibleb-type cytochrome. It is also possible that ascorbate-related redox activity exists at the tonoplast of plant cells.
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Aghion, J., Loser, A. P., Moxhet, A., and Stevens, M. (1992).J. Photochem. Photobiol. B: Biol. 16, 37–46.
Alcain, F. J., Buron, M. I., Villalba, J. M., and Navas, P. (1991).Biochim. Biophys. Acta 1073, 380–385.
Apostal, I., Heinstein, P. F., and Low, P. S. (1989).Plant Physiol. 90, 109–116.
Asard, H., Venken, M., Caubergs, R., Reijnders, W., Oltmann, F. L., and DeGreef, J. A. (1989).Plant Physiol. 90, 1077–1083.
Asard, H., Horemans, N., and Caubergs, R. J. (1992).FEBS Lett. 306, 143–146.
Askerlund, P., and Larsson, C. (1991).Plant Physiol. 96, 1178–1184.
Askerlund, P., Larsson, C., and Widell, S. (1989).Physiol. Plant 76, 123–134.
Askerlund, P., Laurent, P., Nakagawa, H., and Kader, J.-C. (1991).Plant Physiol. 95, 6–13.
Block, G. (1993). InVitamins and Cancer Prevention, Vol. 3 (Bray, G. A., and Ryan, D. H., eds.), LSU Press, Baton Rouge, pp. 247–255.
Böttger, M. (1989). InPlant Membrane Transport: The Current Position (Dainty, J., DeMichelis, M. I., Marré, E., and Rasi-Caldogno, F., eds.), Elsevier, Amsterdam, pp. 55–60.
Bowditch, M. I., and Donaldson, R. P. (1990).Plant Physiol. 94, 531–537.
Bradley, D. J., Kjellbom, P., and Lamb, C. J. (1992).Cell 70, 21–30.
Brightman, A. O., and Morré, D. J. (1990). InOxidoreduction at the Plasma Membrane: Relation to Growth and Transport II (Morré, D. J., Crane, F. L., and Löw, H., eds.), CRC Press, Boca Raton, pp. 85–110.
Chosak, E., Rubinstein, B., and Reinhold, L. (1991).Plant Sci. 77, 163–172.
Dhariwal, K. R., Black, C. D. V., and Levine, M. (1991a).J. Biol. Chem. 266, 12908–12914.
Dharimal, K. R., Shirvan, M., and Levine, M. (1991b).J. Biol. Chem. 266, 5384–5387.
Diliberto, E. J., Jr., Daniels, A. J., and Viveros, O. H. (1991).Am. J. Clin. Nutr. 54, 1163S-1173S.
Dini, L., DiGiulio, A., Pavan, A., Ravagnan, G., and Mossa, G. (1991).Biochim. Biophys. Acta 1062, 108–112.
Donaldson, R. P., and Luster, D. G. (1991).Plant Physiol. 96, 669–674.
Eipper, B. A., Stoffers, L. A., and Mains, R. E. (1992).Annu. Rev. Neurosci. 15, 57–85.
Frei, B., Stocker, R., England, L., and Ames, B. N. (1990). InAntioxidants in Therapy and Preventative Medicine (Emerit, I., Packer, L., and Auclair, C., eds.), Plenum Press, New York, pp. 155–163.
Fukuzawa, K., Seko, T., Minami, K., and Terao, J. (1993).Lipids 28, 497–503.
González-Reyes, J. A., Döring, O., Navas, P., Obst, G., and Böttger, M. (1992).Biochim. Biophys. Acta 1098, 177–183.
Hager, A., Brich, M., Bazlen, I. (1993).Planta 190, 120–126.
Harnadek, G. J., Ries, E. A., Tse, D. G., Fitz, J. S., and Njus, D. (1992).Biochim. Biophys. Acta 1135 280–286.
Hassidim, M., Rubinstein, B., Lerner, H. R., and Reinhold, L. (1987).Plant Physiol. 85 872–875.
Hidalgo, A., García-Herdugo, G., González-Reyes, J. A., Morré, D. J., and Navas, P. (1991).Bot. Gaz. 152 282–288.
Hoffman, K. E., Yanelli, K., and Bridges, K. R. (1991).Am. J. Clin. Nutr. 54 1188S-1192S.
Horemans, N., Asard, H., and Caubergs, R. J. (1994).Plant Physiol.,104 1455–1458.
Jalubar, V., Kelley, P. M., and Njus, D. (1991).J. Biol. Chem. 266 6878–6882.
Kende, H. (1993).Annu. Rev. Plant Physiol. Plant Mol. Biol. 44 283–307.
Kent, U. M., and Fleming, P. J. (1990).J. Biol. Chem. 265 16422–16427.
Luster, D. G., and Buckout, T. J. (1988).Physiol. Plant. 73 339–347.
Luster, D. G., and Buckout, T. J. (1989).Plant Physiol. 91 1014–1019.
Luster, D. G., Bowditch, M. I., Eldridge, K. M., and Donaldson, R. P. (1988).Arch. Biochem. Biophys. 265 50–61.
Matsuda, T., Gemba, T., Baba, A., and Iwata, H. (1990).Brain Res. 532 13–18.
Morel, F., Doussiere, J., and Vignais, P. V. (1991).Eur. J. Biochem. 201 523–546.
Morré, D. J., Sun, I., and Crane, F. L. (1985). InVitamins and Cancer—Human Cancer Prevention by Vitamins and Micronutrients (Meyskens, F. L., and Prasad, K. N., eds.), Humana Press, Clifton, New Jersey, pp. 83–92.
Morré, D. J., Navas, P., Penel, C., and Castillo, F. J. (1986).Protoplasma 133 195–197.
Mossa, G., DiGiulio, A., Dini, L., and Finazzi-Agrò, A. (1989).Biochim. Biophys. Acta 986 310–314.
Navas, P., Estévez, A., Burón, M. I., Villalba, J. M., and Crane, F. L. (1988).Biochem. Biophys. Res. Commun. 154 1029–1034.
Navas, P. (1990). InOxidoreduction at the Plasma Membrane. Relation to Growth and Transport II (Crane, F. L., Morré, D. J., and Löw, H. E., eds.), CRC Press, Boca Raton, pp. 111–120.
Navas, P., Alcain, F. J., Burón, I., Rodriquiz-Alguilera, J.-C., Villalba, J. M., Morré, D. M., and Morré, D. J. (1992).FEBS Lett. 299 223–226.
Niki, E. (1991).Am. J. Clin. Nutr. 54 1119S-1124S.
Njus, D., and Kelley, P. M. (1993).Biochim. Biophys. Acta 1144 235–248.
Nuñez, M.-T., Gaete, V., Watkins, J. A., and Glass, J. (1990).J. Biol. Chem. 265 6688–6692.
Perin, M. S., Fried, V. A., Slaughter, C. A., and Sudhof, T. C. (1988).EMBO J. 7 2697–2703.
Prescott, A. G. (1993).J. Exp. Bot. 44 849–861.
Raguzzi, F., Lesuisse, E., and Crichton, R. R. (1988).FEBS Lett. 231 253–258.
Rubinstein, B., and Stern, A. I. (1991).J. Bioenerg. Biomembr. 23 393–408.
Schwacke, R., and Hager, A. (1992).Planta 187 136–141.
Segal, A. W., and Abo, A. (1993).Trends Biochem. Sci. 18 43–47.
Struglics, A., Fredlund, K. M., Rasmusson, A. G., and Moller, I. M. (1993).Physiol Plant. 88 19–28.
Taiz, L., Gogarten, J. P., Kibak, H., Struve, I., Bernasconi, P., Rausch, T., and Taiz, S. L. (1989). InPlant Membrane Transport: The Current Position (Dainty, J., DeMichelis, M. I., Marre, E., and Rasi-Caldogno, F., eds.), Elsevier, Amsterdam, pp. 131–137.
Takahama, U. (1986).Biochim. Biophys. Acta 882 445–451.
Takahama, U., and Egashira, T. (1991).Phytochemistry 30 73–77.
Tang, X.-Y., Wang, H., Brandt, A. S., and Woodson, W. R. (1993).Plant Mol. Biol. 23 1151–1164.
Villalba, J. M., Canalejo, A., Burón, M. I., Córdoba, F., and Navas, P. (1993).Biochem. Biophys. Res. Commun. 192 707–713.
Watkins, J. A., Nuñez, M.-T., Gaete, V., Alvarez, O., and Glass, J. (1991).J. Membr. Biol. 119 141–149.
Watkins, J. A., Altazan, J. D., Elder, P., Li, C.-Y., Nuñez, M.-T., Cui, X.-X., and Glass, J. (1992).Biochemistry 31 5820–5830.
Wink, M. (1993).J. Exp. Bot. 44 231–246.
Wu, L. N. Y., Sauer, G. R., Genge, B. R., and Wuthier, R. E. (1989).J. Biol. Chem. 264 21346–21355.
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Rubinstein, B. The action of ascorbate in vesicular systems. J Bioenerg Biomembr 26, 385–392 (1994). https://doi.org/10.1007/BF00762779
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DOI: https://doi.org/10.1007/BF00762779