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Journal of Bioenergetics and Biomembranes

, Volume 26, Issue 5, pp 563–571 | Cite as

Relationship between the F0F1-ATPase and the K+-transport system within the membrane of anaerobically grownEscherichia coli. N,N′-dicyclohexylcarbodiimide-sensitive ATPase activity in mutants with defects in K+-transport

  • Armen A. Trchounian
  • Anait V. Vassilian
Original Articles

Abstract

A considerable (2-fold) stimulation of the DCCD-sensitive ATPase activity by K+ or Rb+, but not by Na+, over the range of zero to 100mM was shown in the isolated membranes ofE. coli grown anaerobically in the presence of glucose. This effect was observed only in parent and in thetrkG, but not in thetrkA, trkE, ortrkH mutants. ThetrkG or thetrkH mutant with anunc deletion had a residual ATPase activity not sensitive to DCCD. A stimulation of the DCCD-sensitive ATPase activity by K+ was absent in the membranes from bacteria grown anaerobically in the presence of sodium nitrate. Growth of thetrkG, but not of othertrk mutants, in the medium with moderate K+ activity did not depend on K+ concentration. Under upshock, K+ accumulation was essentially higher in thetrkG mutant than in the othertrk mutant. The K+-stimulated DCCD-sensitive ATPase activity in the membranes isolated from anaerobically grownE. coli has been shown to depend absolutely on both the F0F1 and theTrk system and can be explained by a direct interaction between these transport systems within the membrane of anaerobically grown bacteria with the formation of a single supercomplex functioning as a H+-K+ pump. ThetrkG gene is most probably not functional in anaerobically grown bacteria.

Key words

F0F1 K+-transport ATPase activity interaction between membrane transport systems supercomplex anaerobically grown bacteria 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Armen A. Trchounian
    • 1
  • Anait V. Vassilian
    • 1
  1. 1.Department of BiophysicsBiological Faculty of Yerevan State UniversityYerevanRepublic of Armenia

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