Abstract
Fluorescence quenching by oxygen of cationic [pyrene-(CH2) n N(CH3) +3 ;n=1, 4, and 11] and anionic [pyrene-(CH2) n CO −2 ,n=3, 8, 11, and 15] probes was investigated in erythrocyte plasma membranes (leaky) in order to assess the ability of oxygen molecules to interact with solutes located at different positions in the membrane. The pseudounimolecular quenching rate constants measured increase, both for cationic and anionic probes, whenn increases. These results are interpreted in terms of an increased oxygen solubility toward the center of the membrane interior, and imply that lateral diffusion contributes more than transverse diffusion to total oxygen mobility. For all of the probes considered, quenching rates increase whenn-alkanols are added. The effect observed increases whenn decreases and when the size of then-alkanol alkyl chain increases. Arrhenius-type plots for the quenching rate constants show noticeable downward curvatures. Average (0–40°C) activation energies are ∼6 kcal/mol.
Similar content being viewed by others
Abbreviations
- EPM:
-
erythrocyte plasma membrane
- PMTMA:
-
(1-pyrenyl)methyltrimethyl-ammonium
- PBTMA:
-
4-(1-pyrenyl)butyltrimethylammonium
- PUTMA:
-
11-(1-pyrenyl)-undecyltrimethylammonium
- PB:
-
4-(1-pyrenyl)butanoate
- PN:
-
9-(1-pyrenyl)nonanoate
- PD:
-
12-(1-pyrenyl)dodecanoate
- PHD:
-
16-(1-pyrenyl)hexadecnoate
References
Abuin, E. B., Lissi, E. A., Aravena, D., Zanocco, A., and Macuer, M. (1988).J. Colloid Interface Sci. 122, 201–208.
Afzal, J., Ashlock, S. R., Fung, B. M., and O'Rear, E. A. (1986).J. Phys. Chem. 90, 3019–3022.
Blatt, E., and Sawyer, W. H. (1985).Biochim. Biophys. Acta 822, 43–62.
Dong, D. C., and Winnik, M. A. (1982).Photochem. Photobiol. 35, 17–21.
Fishkoff, S., and Vanderkooi, J. M. (1975).J. Gen. Physiol. 65, 663–676.
Geiger, H. W., and Turro, N. J. (1975).Photochem. Photobiol. 22, 273–276.
Geiger, M. W., and Turro, N. J. (1977).Photochem. Photobiol. 26, 221–224.
Hanahan, D. J., and Ekholm, J. E. (1974).Methods Enzymol. 31, 168–172.
Houslay, M. D., and Stanley, D. D. (1982).Dynamics of Biological Membranes, John Wiley and Sons, New York, pp. 126–128.
Lakowicz, J. R. (1980).J. Biochem. Biophys. Methods 2, 90–119.
Lakowicz, J. R. (1982). InHemoglobin and Oxygen Binding (Cho, C.,et al., eds.), Vol. 1, pp. 443–448.
Lakowicz, J. R., and Weber, G. (1973).Biochemistry 12, 4161–4170.
Lowry, O. H., Bosebrough, N. H., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193, 265–275.
Rowe, E. S., Fernandes, A., and Khalifah, R. G. (1987).Biochem. Biophys. Acta 905, 151–164.
Rubio, M. A., Araya, L., Abuin, E. B., and Lissi, E. A. (1985).An. Asoc. Quim. Argent. 73, 301–309.
Thulborn, K. R., and Sawyer, W. H. (1978).Biochim. Biophys. Acta 471, 125–140.
Tristman, S. N., and Moynihan, M. M. (1981).Biochim. Biophys. Acta 898, 109–120.
Tsuchida, E., Nishida, H., and Yuasa, M. (1984).J. Chem. Soc. [D], 96–98.
Turro, N. J., Aikawa, M., and Yekta, A. (1979).Chem. Phys. Lett. 64, 473–478.
Vachon, A., Lecomte, C., Berleur, F., Roman, V., Fatome, M., and Braquet, P. (1987).J. Chem. Soc. Faraday Trans. 1,83, 177–190.
Vanderkooi, J., and McLaughlin, A. (1976). InBiochemical Fluorescence Concepts (Chen, R., and Edelhoch, H., eds.), Ch. 19, pp. 737–765.
Vaughan, W., and Weber, G. (1970).Biochemistry 10, 464–470.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lissi, E.A., Caceres, T. Oxygen diffusion-concentration in erythrocyte plasma membranes studied by the fluorescence quenching of anionic and cationic pyrene derivatives. J Bioenerg Biomembr 21, 375–385 (1989). https://doi.org/10.1007/BF00762728
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00762728