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Analysis of the H+/sugar symport in yeast under conditions of depolarized plasma membrane

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Abstract

H+/sugar symport in the obligatory aerobic yeastRhodotorula glutinis was analyzed under conditions where the plasma membrane was selectively depolarized by the lipophilic cation tetraphenylphosphonium (TPP+). Control experiments showed that this treatment did not impair the transmembrane ΔpH, the cell energy charge, and the function of plasma membrane H+-ATPase. The kinetic data were fitted to elementary functions derived from a model constructed on the basis of some simplifying premises for ordered (either C + H+ + S or C + S + H+) and random reaction mechanisms. In addition, the comparison of the kinetic parameters in fully energized and depolarized cells provided information about the free carrier charge. It was concluded that the binding sequence of formation of the ternary carrier/H+/substrate complex follows a random mechanism and that the carrier bears a negative charge.

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References

  • Bakker, R., Borst-Pauwels, G. W. F. H., and Dobbelmann, J. (1986).Biochim. Biophys. Acta 861, 205–209.

    Google Scholar 

  • Dixon, W. J. (ed.) (1983).BMDP Statistical Software, University of California Press, Berkeley.

    Google Scholar 

  • Geck, P. (1978). Habilitationsschrift. Gustav Embden Center for Physiological Chemistry, University of Frankfurt, Frankfurt a.M.

    Google Scholar 

  • Hauer, R., and Höfer, M. (1978).J. Membr. Biol. 43, 335–349.

    Google Scholar 

  • Hauer, R., Uhlemann, G., Neumann, J., and Höfer, M. (1981).Biochim. Biophys. Acta 649, 680–690.

    Google Scholar 

  • Heinz, E., Geck, P., and Wilbrandt, W. (1972).Biochim. Biophys. Acta 255, 442–461.

    Google Scholar 

  • Heller, K. B., and Höfer, M. (1975).J. Membr. Biol. 21, 261–271.

    Google Scholar 

  • Höfer, M. (1989). InMethods in Enzymology, Part M: Biologicl Transport (Fleischer, S., and Fleischer, B., eds.), Vol. 4, Academic Press, Orlando, FL, (in press).

    Google Scholar 

  • Höfer, M., and Künemund, A. (1984).Biochem. J. 225, 815–819.

    Google Scholar 

  • Höfer, M., and Misra, P. C. (1978).Biochem. J. 172, 15–22.

    Google Scholar 

  • Höfer, M., and Nassar, F. R. (1987).J. Gen. Microbiol. 133, 2163–2172.

    Google Scholar 

  • Höfer, M., and Novacky, A. (1986).Biochim. Biophys. Acta 862, 372–378.

    Google Scholar 

  • Höfer, M., Nicolay, K., and Robillard, G. (1985).J. Bioenerg. Biomembr. 17, 175–182.

    Google Scholar 

  • Jauch, P., and Läuger, P. (1986).J. Membr. Biol. 94, 117–127.

    Google Scholar 

  • Jaworek, D., and Welsch, J. (1985). InMethods of Enzymatic Analysis (Bergmeyer, H. U., ed.), Vol. 7, VCH Verlagsgesellschaft, Weinheim, FRG, pp. 365–370.

    Google Scholar 

  • Kotyk, A., and Höfer, M. (1965).Biochim. Biophys. Acta 102, 410–422.

    Google Scholar 

  • Kunemund, A., and Höfer, M. (1983).Biochim. Biophys. Acta 735, 203–210.

    Google Scholar 

  • Lichtenberg, H. C., Glebeler, H., and Höfer, M. (1988).J. Membr. Biol.

  • Mahlberg, D., Höfer, M., and Täuber, A. (1985).J. Gen. Microbiol. 131, 479–485.

    Google Scholar 

  • Misra, P. C., and Höfer, M. (1975).FEBS Lett. 52, 95–99.

    Google Scholar 

  • Neame, K. D., and Homewood, C. A. (1974).Introduction to Liquid Scintillation Counting, Butterworths, London.

    Google Scholar 

  • Niemietz, C., and Höfer, M. (1984).J. Membr. Biol. 80, 235–242.

    Google Scholar 

  • Sanders, D. (1986).J. Membr. Biol. 90, 67–87.

    Google Scholar 

  • Sanders, D., Hansen, U. P., Gradmann, D., and Slayman, C. L. (1984).J. Membr. Biol. 77, 123–152.

    Google Scholar 

  • Taghikhani, M., Lavi, L. W., Woost, P. G., and Griffin, C. (1984).Biochim. Biophys. Acta 803, 278–283.

    Google Scholar 

  • Trautschold, I., Lamprecht, W., and Schweitzer, G. (1985). InMethods of Enzymatic Analysis (Bergmeyer, H. U., ed.), Vol. 7, VCH Verlagsgesellschaft, Weinheim, FRG, pp. 346–357.

    Google Scholar 

  • Turner, R. J. (1981).Biochim. Biophys. Acta 649, 269–280.

    Google Scholar 

  • Turner, R. J. (1985).J. Membr. Biol. 88, 77–83.

    Google Scholar 

  • Umbreit, W. W. (1949).Manometric Measurement, Burgess, Minneapolis.

    Google Scholar 

  • Vacata, V., Kotyk, A., and Sigler, K. (1981).Biochim. Biophys. Acta 643, 265–268.

    Google Scholar 

  • Van den Broek, P. J. A., and van Steveninck, J. (1980).Biochim. Biophys. Acta 602, 419–432.

    Google Scholar 

  • Woost, P. G., and Griffin, C. C. (1984).Biochim. Biophys. Acta 803, 284–289.

    Google Scholar 

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Authors and Affiliations

  1. Botanisches Institut der Universität, Kirschallee I, 5300, Bonn 1, Germany

    Jörg Severin, Peter Langel & Milan Höfer

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  1. Jörg Severin
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  2. Peter Langel
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  3. Milan Höfer
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Severin, J., Langel, P. & Höfer, M. Analysis of the H+/sugar symport in yeast under conditions of depolarized plasma membrane. J Bioenerg Biomembr 21, 321–334 (1989). https://doi.org/10.1007/BF00762724

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  • DOI: https://doi.org/10.1007/BF00762724

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