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Respiratory Na+ pump and Na+-dependent energetics inVibrio alginolyticus

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Abstract

The marine bacteriumVibrio alginolyticus was found to possess the respiratory Na+ pump that generates an electrochemical potential of Na+, which plays a central role in bioenergetics ofV. alginolyticus, as a direct result of respiration. Mutants defective in the Na+ pump revealed that one of the two kinds of NADH: quinone oxidoreductase requires Na+ for activity and functions as the Na+ pump. The Na+ pump composed of three subunits was purified and reconstituted into liposomes. Generation of membrane potential by the reconstituted proteoliposomes required Na+. The respiratory Na+ pump coupled to the NADH: quinone oxidoreductase was found in wide varieties of Gramnegative marine bacteria belonging to the generaAlcaligenes, Alteromonas, andVibrio, and showed a striking similarity in the mode of electron transfer and enzymic properties. Na+ extrusion seemed to be coupled to a dismutation reaction, which leads to the formation of quinol and quinone from semi-quinone radical.

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Authors and Affiliations

  1. Institute of Applied Microbiology, University of Tokyo, 1-1-1 Yayoi, Bunkyo, 113, Tokyo, Japan

    Hajime Tokuda

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  1. Hajime Tokuda
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Tokuda, H. Respiratory Na+ pump and Na+-dependent energetics inVibrio alginolyticus . J Bioenerg Biomembr 21, 693–704 (1989). https://doi.org/10.1007/BF00762687

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  • DOI: https://doi.org/10.1007/BF00762687

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