Journal of Bioenergetics and Biomembranes

, Volume 25, Issue 3, pp 307–312 | Cite as

Inhibition of the mitochondrial calcium uniporter by antibodies against a 40-kDa glycorproteinT

  • Nils-Erik L. Saris
  • Tatjana V. Sirota
  • Ismo Virtanen
  • Kaija Niva
  • Timo Penttilä
  • Ludmila P. Dolgachova
  • Galina D. Mironova
Original Articles


Polyclonal rabbit antibodies against a Ca2+-binding mitochondrial glycoprotein were found to inhibit the uniporter-mediated transport of Ca2+ in mitoplasts prepared from rat liver mitochondria. Spermine, a modulator of the uniporter, decreased the inhibition. This glycoprotein ofM r 40,000, isolated from beef heart mitochondria and earlier shown to form Ca2+-conducting channels in black-lipid membranes, thus is a good candidate for being a component of the uniporter. Antibody-IgG was found to specifically bind to mitochondria in human fibroblasts.

Key words

Calcium transport fibroblast glycoprotein heart mitochondria spermine uniporter 


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Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • Nils-Erik L. Saris
    • 1
  • Tatjana V. Sirota
    • 2
  • Ismo Virtanen
    • 3
  • Kaija Niva
    • 1
  • Timo Penttilä
    • 1
  • Ludmila P. Dolgachova
    • 4
  • Galina D. Mironova
    • 2
  1. 1.Department of Medical ChemistryUniversity of HelsinkiHelsinkiFinland
  2. 2.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchinoRussia
  3. 3.Department of AnatomyUniversity of HelsinkiHelsinkiFinland
  4. 4.Institute of Theoretical and Experimental BiophysicsAcademy of Sciences of RussiaRussia

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