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Cytotechnology

, Volume 15, Issue 1–3, pp 209–215 | Cite as

Effect of lipid supplements on the production and glycosylation of recombinant interferon-γ expressed in CHO cells

  • Nigel Jenkins
  • Paula Castro
  • Sunitha Menon
  • Andrew Ison
  • Alan Bull
Article

Abstract

The effects of lipids on the glycosylation of recombinant human interferon-γ expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-γ deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-γ glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.

Key words

Glycosylation recombinant interferon CHO cells lipids 

Abbreviations

BSA

bovine serum albumin

CHO

chinese hamster ovary

DHFR

dihydrofolate reductase

FCS

foetal calf serum

IFN-γ

human interferon-gamma

qIFN

specific interferon production rate

μ

specific growth rate

2N

doubly-gycosylated

1N

singly-glycosylated

ON

non-glycosylated

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Nigel Jenkins
    • 2
  • Paula Castro
    • 1
  • Sunitha Menon
    • 1
  • Andrew Ison
    • 2
  • Alan Bull
    • 1
  1. 1.Research School of BiosciencesUniversity of KentCanterburyUK
  2. 2.SERC Advanced Centre for Biochemical Engineering, University CollegeLondon UniversityLondonUK

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