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Cytotechnology

, Volume 15, Issue 1–3, pp 111–116 | Cite as

Low temperature cultivation — A step towards process optimisation

  • Ralf Weidemann
  • Andreas Ludwig
  • Gerlinde Kretzmer
Article

Abstract

Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37°C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.

Key words

Adherent animal cells glucose lactate productivity temperature 

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References

  1. Bloemkolk J-W, Gray MR, Merchant F and Mosmann TR (1992) Effect of temperature on hybridoma cell cycle and Mab production. Biotechnol. and Bioeng. 40:427–431.Google Scholar
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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Ralf Weidemann
    • 1
  • Andreas Ludwig
    • 1
  • Gerlinde Kretzmer
    • 1
  1. 1.Institut für Technische ChemieHannoverGermany

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