Abstract
The relationship between growth and interferon-γ (IFN-γ) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. TheK s and μ max values were found to be 0.771% (v/v) serum and 0.031 h−1 respectively. The average specific IFN-γ production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-γ production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.
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Leelavatcharamas, V., Emery, A.N. & Al-Rubeai, M. Growth and interferon-γ production in batch culture of CHO cells. Cytotechnology 15, 65–71 (1994). https://doi.org/10.1007/BF00762380
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DOI: https://doi.org/10.1007/BF00762380