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Cytotechnology

, Volume 15, Issue 1–3, pp 65–71 | Cite as

Growth and interferon-γ production in batch culture of CHO cells

  • V. Leelavatcharamas
  • A. N. Emery
  • M. Al-Rubeai
Article

Abstract

The relationship between growth and interferon-γ (IFN-γ) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. TheKs and μ max values were found to be 0.771% (v/v) serum and 0.031 h−1 respectively. The average specific IFN-γ production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-γ production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.

Key words

Animal cell culture interferon CHO growth rate batch culture cell cycle 

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References

  1. Al-Rubeai M, Emery AN, Chalder S, Jan DC (1992) Specific monoclonal antibody productivity and the cell cycle- comparisons of batch, continuous and perfusion cultures. Cytotechnology 9: 85–97.Google Scholar
  2. Buntemeyer H, Wallerius C, Lehmann J (1992) Optimal medium use for continuous high density perfusion processes. Cytotechnology 9: 59–67.Google Scholar
  3. Dalili M and Ollis DF (1989). Transient kinetics of hybridoma growth and monoclonal antobody production in serum-limited cultures. Biotechnol. Bioeng. 33: 984–990.Google Scholar
  4. Fawcett JK, and Scott JE (1960) A rapid and precise method for the determination of urea J. Clin. Path. 13: 156–159.Google Scholar
  5. Field RP, Brand H, Renner GL, Robertson HA, Boraston R (1991) Production of a chimeric antibody for tumour imaging and therapy from chinese hamster overy (CHO) and myeloma cells. In: Production of biologicals from animal cells in culture. (Eds.) Spier RE, Griffiths JB, Meignier B, Butterworth-Heinemann, Oxford pp. 742–744.Google Scholar
  6. Geaugey V, Duval D, Geahel I, Marc A, Engasser JM (1989) Infulence of amino acids on hybridoma cell viability and antibody secretion. Cytotechnology 2: 119–129.Google Scholar
  7. Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strange PG, Bull AT (1991) Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture. Appl. Microbiol. 34: 559–564.Google Scholar
  8. Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strange PG, Tong JM, Bull AT (1992) Glucose-limited chemostat culture of chinese hamster overy cells producing recombinant human interferon-γ. Biotechnology and Bioengineering 29: 327–335.Google Scholar
  9. Hayter PM, Curling EMA, Gould ML, Baines AJ, Jenkins N, Salmon I, Strange PG, Bull AT (1993) The effect of the dilution rate on the CHO cell physiology and recombinant interferon-γ production in glucose-limited chemostat culture. Biotechnology and Bioengineering 42: 1077–1085.Google Scholar
  10. Hild HM, Emery AN, and Al-Rubeai M, (1992) The effect of pH, temperature, serum concentration and media composition on the growth of insect cells In: Workshop on baculovirus and recombinant protein production processes. Edited by JM Vlak, EJ, Schlaeger and AR, Bernard, March 29–April 1, Interlaken, Switzerland.Google Scholar
  11. Jan DC, Al-Rubeai M, Emery AN (1991) Towards low-cost mediumn formulation for intensive animal cell culture. In: (eds.) Huyghebaert A, and Vandamme E, Upstream and downstream processing in biotechnology III (pp. 1.17–1.26) Royal Flemish Society of Engineers, Antwerpen.Google Scholar
  12. Leelavatcharamas V, (1994) Growth, cell cycle and interferon-γ production of CHO cells in batch culture M. Phil. thesis. School of chemical engineering. University of Birmingham.Google Scholar
  13. Luan YT, Mutharasan R and Magee WE, (1987) Strategies to extend longevity of hybridomas in culture and promote yield of monoclonal antibodies Biotechn. Lett., 9: 691–696.Google Scholar
  14. Reid S, Saxena V, Greenfield PF, Weiss SA (1992) Applicability of fed batch processes for improving recombinant protein production by the baculovirus expression system. In: Spier RE, Griffiths JB, and MacDonald C, Animal cell Technology: developments, processes and products (eds.) pp. 276–281. Butterworth-Heinemann, Oxford.Google Scholar
  15. Thomas JN, (1990) Mammalian cell physiology in: (eds.) Lubiniecki AS, p 119, Large-scale mammalian cell culture technology, Marcel Dekker Inc. New York.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • V. Leelavatcharamas
    • 1
  • A. N. Emery
    • 1
  • M. Al-Rubeai
    • 1
  1. 1.BBSRC Centre for Biochemical Engineering, School of Chemical EngineeringThe University of BirminghamBirminghamUK

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