, Volume 15, Issue 1–3, pp 65–71 | Cite as

Growth and interferon-γ production in batch culture of CHO cells

  • V. Leelavatcharamas
  • A. N. Emery
  • M. Al-Rubeai


The relationship between growth and interferon-γ (IFN-γ) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. TheKs and μ max values were found to be 0.771% (v/v) serum and 0.031 h−1 respectively. The average specific IFN-γ production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-γ production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.

Key words

Animal cell culture interferon CHO growth rate batch culture cell cycle 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • V. Leelavatcharamas
    • 1
  • A. N. Emery
    • 1
  • M. Al-Rubeai
    • 1
  1. 1.BBSRC Centre for Biochemical Engineering, School of Chemical EngineeringThe University of BirminghamBirminghamUK

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