Skip to main content
Log in

Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production

  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The effects of linoleic acid and bovine serum albumin on hybridoma cell growth and antibody production were investigated. In dish cultivation, linoleic acid on its own promoted cell growth when used at concentrations below 50 mg L−1, but strongly inhibited growth at a concentration of 100 mg L−1 on more. However, linoleic acid bound to bovine serum albumin did not inhibit cell growth, even at a concentration as high as 100 mg L−1. Also, linoleic acid did not affect the specific antibody production rate, with or without bovine serum albumin. In order to elucidate the enhancement of antibody production by bovine serum albumin, fractions were prepared by ultrafiltration (98% molecular weight cut-offs, 50,000 and 17,000) and the effects of the fractionation on antibody production were studied in batch cultivation. The high-molecular-weight fraction (≧50,000) promoted antibody production whereas the low-molecular-weight fraction (≦17,000) inhibited it. In continuous cultivation, the high-molecular-weight fraction was also found to enhance antibody production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bandyopadhyay GK, Imagawa W, Wallace D and Nandi S (1987) Linoleate metabolites enhance thein vitro proliferative response of mouse mammary epithelial cells to epidermal growth factor. J. Biol. Chem. 262: 2750–2756.

    Google Scholar 

  • Chen RF (1967) Removal of fatty acids from serum albumin by charcoal treatment J. Biol. Chem. 242: 173–181.

    Google Scholar 

  • Halliwell B (1988) Albumin — An important extracellular antioxidant? Biochem. Pharmacol. 37: 569–571.

    Google Scholar 

  • Johnson AR and Stocks RB (1971) Gas-liquid chromatography of lipids. In: Biochemistry and Methodology of Lipids, pp. 195–218.

  • Murakami H, Masui H, Sato GH, Sueoka N, Chow TP and Sueoka TK (1982) Growth of hybridoma cells in serum-free medium: Ethanolamine is an essential component. Proc. Natl. Acad. Sci. USA 79: 1158–1162.

    Google Scholar 

  • Nilausen K (1978) Role of fatty acids in growth-promoting effect of serum albumin on hamster cellsin vitro. Journal of Cellular Physiology 96: 1–14.

    Google Scholar 

  • Omasa T, Kobayashi M, Nishikawa T, Shioya S, Suga K, Uemura S, Kitani Y, and Imamura Y (1991) Hybridoma culture in hollowfiber system — The effects of growth factors —. In: R. Sasaki and K. Ikura (eds.) Animal cell culture and production of biologicals. (pp. 229–236) Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Omasa T, Higashiyama K, Shioya S, and Suga K (1992a) Effects of lactate concentrations on hybridoma culture in lactate-controlled fed-batch operation Biotechnol.Bioeng. 39: 556–564.

    Google Scholar 

  • Omasa T, Ishimoto M, Higashiyama K, Shioya S, and Suga K (1992b) The enhancement of specific antibody production rate in glucose- and glutamine-controlled fed-batch culture. Cytotechnology 8: 75–84.

    Google Scholar 

  • Rockwell GA, Sato GH, and McClure DB (1980) The growth requirements of SV40 virus transformed Balb/c-3T3 cells in serum-free monolayer culture. Journal of Cellular Physiology 103: 323–331.

    Google Scholar 

  • Schmid G, Zilg H, Eberhard U, and Johannsen R (1991) Effect of free fatty acids and phospholipids on growth of and product formation by recombinant baby hamster kidney (rBHK) and Chinese hamster ovary (rCHO) cells in culture. J. of Biotechnology 17: 155–167.

    Google Scholar 

  • Spector AA (1975) Fatty acid binding to plasma albumin. J. Lipid Res. 16: 165–179

    Google Scholar 

  • Spieker-Polet H, and Polet H (1981) Requirement of a combination of a saturated and an unsaturated free fatty acid and a fatty acid carrier protein forin vitro growth of lymphocytes. J. Immunol. 126: 949–954.

    Google Scholar 

  • Sugahara T, Shirahata S, Yamada K and Murakami H (1991a) Purification of immunoglobulin production stimulating factor IIα derived from Namalwa cells. Cytotechnology 5: 255–263.

    Google Scholar 

  • Sugahara T, Shirahata S, Akiyoshi K, Isobe T, Okuyama T and Murakami H (1991b) Immunoglobulin production stimulating factor-IIα (TPSF-IIα) is glyceraldehyde-3-phosphate dehydrogenase like protein. Cytotechnology 6: 115–120.

    Google Scholar 

  • Yamada K, Ikeda I, Nakajima H, Shirahata S and Murakami H (1991) Stimulation of proliferation and immunoglobulin production of human-human hybridoma by various types of caseins and their protease digests. Cytotechnology 5: 279–285.

    Google Scholar 

  • Yamane I, Murakami O, and Kato M (1978) Role of bovine serum albumin in a serum-free suspension cell culture medium (38823). Proc. Soc. Exp. Biol. Med. 149: 439–442.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kobayashi, M., Kato, S., Omasa, T. et al. Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production. Cytotechnology 15, 51–56 (1994). https://doi.org/10.1007/BF00762378

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00762378

Key words

Navigation