Skip to main content
SpringerLink
Log in

Structural and functional aspects of the respiratory chain of synaptic and nonsynaptic mitochondria derived from selected brain regions

  • Research Articles
  • Published:
Journal of Bioenergetics and Biomembranes Aims and scope Submit manuscript

Abstract

Studies on brain mitochondria are complicated by the regional, cellular, and subcellular heterogeneity of the central nervous system. This study was performed using synaptic and nonsynaptic mitochondria obtained from cortex, hippocampus, and striatum of male Sprague-Dawley rats (3 months old). Ubiquinone content, detected by HPLC analysis, was about 1.5 nmol/mg protein with an approximate CoQ9/CoQ10 molecular ratio of 2:1. The activities of several respiratory chain complexes were also studied (succinate-cyt.c reductase, NADH-cyt.c reductase, succinate-DCIP, ubiquinol2-cyt.c reductase, and cytochrome oxidase), and generally found to be higher in mitochondria from cortex than from other regions. Study of the activities of some of these enzymes vs. 1/T (Arrhenius plots) showed a straight line with an activation energy between 7 and 10 kcal/mol in all the three areas considered. Only CoQ2H2-cyt.c reductase activity revealed a biphasic temperature dependence. Also anisotropy (as fluorescence polarization) of the hydrophobic probe DPH showed a deviation from linearity; the break points for both enzymatic activity and anisotropy were found at about 23–24°C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baracca, A., Solaini, G., Parenti-Castelli, G., and Lenaz, G. (1984).Ital. J. Biochem. 33, 193A-194A.

    Google Scholar 

  • Baracca, A., Solaini, G., Parenti-Castelli, G., Curatola, G., and Lenaz, G. (1986a).Cell Biol. Int. Rep. 10, 194.

    Google Scholar 

  • Baracca, A., Curatola, G., Parenti-Castelli, G., and Solaini, G. (1986b).Biochem. Biophys. Res. Commun. 136, 891–898.

    Google Scholar 

  • Battino, M., Fahmy, T., and Lenaz, G. (1986a).Biochim. Biophys. Acta 851, 377–384.

    Google Scholar 

  • Battino, M., Rugolo, M., Romeo, G., and Lenaz, G. (1986b).FEBS Lett. 199, 155–158.

    Google Scholar 

  • Battino, M., Castelluccio, C., Pilarska, M., Sarzala, M. G., and Lenaz, G. (1986c).Cell Biol. Int. Rep. 10, 154.

    Google Scholar 

  • Battino, M., Bertoli, E., Marchetti, M., Bovina, C., Formiggini, G., Villa, R. F., Gorini, A., Benzi, G., and Lenaz, G. (1988).Abstracts 14th International Congress of Biochemistry, Prague, p. 175.

  • Battino, M., Bertoli, E., Raciti, G., Lenaz, G., and Cambria, A. (1989a).Ital. J. Biochem. 38, 66A-67A.

    Google Scholar 

  • Battino, M., Bertoli, E., and Lenaz, G. (1989b).Ital. J. Biochem. 38, 195A-196A.

    Google Scholar 

  • Battino, M., Bertoli, E., Formiggini, G., Gorini, A., Villa, R. F., and Lenaz, G. (1990). InHighlights in Ubiquinone Research, (Lenaz, G., Barnabei, O., Rabbi, A., and Battino, M., eds.), Taylor and Francis, London, pp. 178–181.

    Google Scholar 

  • Beece, D., Eisenstein, L., Fraunfelder, H., Good, D., Marden, M. C., Reinisch, L., Reynolds, A. H., Sorensen, L. B., and Yue, R. T. (1980).Biochemistry 19, 5147–5157.

    Google Scholar 

  • Benzi, G. (1979).Interdiscipl. Top. Gerontol. 15, 104–120.

    Google Scholar 

  • Benzi, G. (1981). InHandbook of Neurochemistry (Lajtha, A., ed.), Plenum Press, New York, pp. 531–542.

    Google Scholar 

  • Benzi, G., Arrigoni, E., Dagani, F., Marzatico, F., Curti, D., Raimondo, S., Dossena, M., Polgatti, M., and Villa, R. F. (1980).Exp. Gerontol. 15, 593–603.

    Google Scholar 

  • Benzi, G., Pastoris, O., and Villa, R. F. (1988a).Neurochem. Res. 13, 467–478.

    Google Scholar 

  • Benzi, G., Pastoris, O., Marzatico, F., and Villa, R. F. (1988b).Neurobiol. Aging 9, 371–375.

    Google Scholar 

  • Benzi, G., Pastoris, O., Marzatico, F., and Villa, R. F. (1989a).Neurochem. Res. 14, 573–581.

    Google Scholar 

  • Benzi, G., Marzatico, F., Pastoris, O., and Villa, R. F. (1989b).Exp. Gerontol. 24, 137–148.

    Google Scholar 

  • Bertoli, E., Parenti-Castelli, G., Landi, L., Sechi, A. M., and Lenaz, G. (1973).J. Bioenerg. 4, 591–598.

    Google Scholar 

  • Biosca, J. A., Travers, F., and Borman, T. E. (1983).FEBS Lett. 153, 217–220.

    Google Scholar 

  • Blokhuis, G. G. D., and Veldstra, H. (1970).FEBS Lett. 11, 197–199.

    Google Scholar 

  • Booth, R. F. G., and Clark, J. B. (1978).Biochem. J. 176, 365–370.

    Google Scholar 

  • Brasitus, T. A., and Schachter, D. (1980a).Biochim. Biophys. Acta 630, 152–156.

    Google Scholar 

  • Brasitus, T. A., and Schachter, D. (1980b).Biochemistry 19, 2763–2769.

    Google Scholar 

  • Brasitus, T. A., Tall, A. R., and Schachter, D. (1980).Biochemistry 19, 1256–1261.

    Google Scholar 

  • Brasitus, T. A., Yeh, K., Halt, P. R., and Schachter, D. (1984).Biochim. Biophys. Acta 778, 341–348.

    Google Scholar 

  • Calanni-Rindina, F., Baracca, A., Solaini, G., Rabbi, A., and Parenti-Castelli, G. (1986).FEBS Lett. 198, 353–356.

    Google Scholar 

  • Clark, J. B., and Nicklas, W. J. (1970).J. Biol. Chem. 245, 4724–4731.

    Google Scholar 

  • Curatola, G., Fiorini, R. M., Solaini, G., Baracca, A., Parenti-Castelli, G., and Lenaz, G. (1983).FEBS Lett. 155, 131–134.

    Google Scholar 

  • Dean, W. L., and Tanford, C. (1978).Biochemistry 17, 1683–1690.

    Google Scholar 

  • Degli Esposti, M., Ferri, E., and Lenaz, G. (1981).Ital. J. Biochem. 30, 437–452.

    Google Scholar 

  • Degli Esposti, M., and Lenaz, G. (1982a).Biochim. Biophys. Acta 682, 189–200.

    Google Scholar 

  • Degli Esposti, M., and Lenaz, G. (1982b).Arch. Biochem. Biophys. 216, 727–735.

    Google Scholar 

  • Dennis, S. C., Lai, J. C. K., and Clark, J. B. (1977).Biochem. J. 164, 727–736.

    Google Scholar 

  • Deshmukh, D. R., Owen, O. E., and Patel, M. S. (1980).J. Neurochem. 34, 1219–1224.

    Google Scholar 

  • Dienel, G., Ryder, E., and Greengard, O. (1977).Biochim. Biophys. Acta 496, 484–494.

    Google Scholar 

  • Dixon, M., and Webb, E. C. (1964).Enzymes, Longmans, London.

    Google Scholar 

  • Drost-Hansen, W. (1969).Chem. Phys. Lett. 2, 647–657.

    Google Scholar 

  • Gutman, M. (1985). InCoenzyme Q (Lenaz, G., ed.), Wiley, London, pp. 215–234.

    Google Scholar 

  • Hajos, F., and Kerpel-Fronius, S. (1969).Exp. Brain Res. 8, 66–78.

    Google Scholar 

  • Hajos, F., and Kerpel-Fronius, S. (1971).J. Cell. Biol. 51, 216–222.

    Google Scholar 

  • Hansford, R. G. (1983).Biochim. Biophys. Acta 726, 41–80.

    Google Scholar 

  • Hatefi, Y., and Stiggall, D. L. (1978).Methods Enzymol. 53, 21–27.

    Google Scholar 

  • Heyn, M. P. (1979).FEBS Lett. 108, 359–364.

    Google Scholar 

  • Jähnig, F. (1979).Proc. Natl. Acad. Sci. USA 76, 6361–6365.

    Google Scholar 

  • Kaizu, T., Kirino, Y., and Shimizu, H. (1980).J. Biochem. 88, 1837–1843.

    Google Scholar 

  • Kröger, A. (1978).Methods Enzymol. 53, 579–591.

    Google Scholar 

  • Lai, J. C. K., and Clark, J. B. (1976).Biochem. J. 154, 423–432.

    Google Scholar 

  • Lai, J. C. K., Walsh, J. M., Dennis, S. C., and Clark, J. B. (1977).J. Neurochem. 28, 625–631.

    Google Scholar 

  • Lai, J. C. K., and Clark, J. B. (1979).Methods Enzymol. 55, 51–60.

    Google Scholar 

  • Lenaz, G. (1979).Subcell. Biochem. 6, 233–337.

    Google Scholar 

  • Lenaz, G. (1987). InBiomembrane and Receptor Mechanisms (Bertoli, E., Chapman, D., Cambria, A., and Scapagnini, U., eds.), Fidia Research Series, Vol. 7, Liviana Press, Padova/Springer-Verlag, Berlin, pp. 113–133.

    Google Scholar 

  • Lenaz, G. (1988). InBiomembranes (Benga, Gh., and Tager, J. M., eds.), Springer-Verlag, Berlin, pp. 13–49.

    Google Scholar 

  • Lenaz, G., and Degli Esposti, M. (1985). InCoenzyme Q (Lenaz, G., ed.), Wiley, London, pp. 83–105.

    Google Scholar 

  • Lenaz, G., and Parenti-Castelli, G. (1985). InBiological Membranes, Vol. I (Benga, G., ed.), CRC Press, Boca Raton, pp. 93–136.

    Google Scholar 

  • Lenaz, G., Baracca, A., Calanni-Rindina, F., Parenti-Castelli, G., Solaini, G., and Curatola, G. (1984). InH + ATPase (ATP Synthase): Structure, Function, Biogenesis. The F 0 F 1 Complex of Coupling Membranes (Papa, S., Altendorf, K., Ernster, L., and Packer, L., eds.), Adriatica Editrice, Bari, pp. 325–326.

    Google Scholar 

  • Lenaz, G., Battino, M., Degli Esposti, M., Fato, R., and Parenti-Castelli, G. (1986). InBiomedical and Clinical Aspects of coenzyme Q (Folkers, K., and Yamamura, Y., eds.), Vol. 5, Elsevier, North-Holland, pp. 73–84.

    Google Scholar 

  • Lenaz, G., Fato, R., Battino, M., Castelluccio, C., Parenti-Castelli, G., and Degli Esposti, M. (1987a).Advances in Myochemistry: 1 (Benzi, G., ed.), John Libbey Eurotext Ltd., pp. 63–73.

  • Lenaz, G., Fato, R., Battino, M., Mandrioli, E., and Parenti-Castelli, G. (1987b). InBioenergetics: Structure and Functions of Energy-Transducing Systems (Ozawa, T., and Papa, S., eds.), Japan Sci. Soc. Press, Tokyo/Springer-Verlag, Berlin, pp. 49–61.

    Google Scholar 

  • Leong, S. F., Lai, J. C. K., Lim, L., and Clark, J. B. (1984).J. Neurochem. 42, 1306–1312.

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193, 265–275.

    Google Scholar 

  • Mayer, H., and Isler, D. (1971).Methods Enzymol. 18c, 182–213.

    Google Scholar 

  • McMurchie, E. J., Gibson, R. A., Aberywardena, M. Y., and Charnock, J. S. (1983).Biochim. Biophys. Acta 727, 163–169.

    Google Scholar 

  • Munn, E. A. (1974). InThe Structure of Mitochondria, Academic Press, London.

    Google Scholar 

  • Nicholls, P. (1976).Biochim. Biophys. Acta 430, 30–45.

    Google Scholar 

  • Owen, F., Bourne, R. C., Lai, J. C. K., and Williams, R. (1977).Biochem. Pharmacol. 26, 289–292.

    Google Scholar 

  • Parenti-Castelli, G., Sechi, A. M., Landi, L., Cabrini, L., Mascarello, S., and Lenaz, G. (1979).Biochim. Biophys. Acta 547, 161–169.

    Google Scholar 

  • Parenti-Castelli, G., Baracca, A., Fato, R., and Rabbi, A. (1983).Biochem. Biophys. Res. Commun. 111, 366–372.

    Google Scholar 

  • Parenti-Castelli, G., Fato, R., Battino, M., Castelluccio, C., and Lenaz, G. (1987).Chem. Scr. 27, 161–166.

    Google Scholar 

  • Parsons, P., and Basford, R. E. (1967).J. Neurochem. 14, 823–840.

    Google Scholar 

  • Raison, J. K. (1973).J. Bioenerg. 4, 285–310.

    Google Scholar 

  • Reijnierse, G. L. A., Veldstra, H., and Van Den Berg, C. J. (1975).Biochem. J. 152, 469–475.

    Google Scholar 

  • Rieske, J. S. (1967).Methods Enzymol. 10, 239–245.

    Google Scholar 

  • Sacktor, B., and Packer, L. (1962).J. Neurochem. 9, 371–382.

    Google Scholar 

  • Salganicoff, L., and Koeppe, R. E. (1968).J. Biol. Chem. 243, 2416–2420.

    Google Scholar 

  • Sechi, A. M., Landi, L., Bertoi, E., Parentil-Castelli, G., and Lenaz, G. (1973).J. Bioenerg. 5, 73–83.

    Google Scholar 

  • Shinitzky, M., and Inbar, M. (1976).Biochim. Biophys. Acta 433, 133–149.

    Google Scholar 

  • Shinitzky, M., and Barenholz, Y. (1978).Biochim. Biophys. Acta 515, 367–394.

    Google Scholar 

  • Smith, L., Davies, H. C., and Nava, M. (1974).J. Biol. Chem. 249, 2904–2910.

    Google Scholar 

  • Solaini, G., Baracca, A., Parenti-Castelli, G., and Lenaz, G. (1984).J. Bioenerg. Biomembr. 16, 391–406.

    Google Scholar 

  • Takada, M., Ikenoya, S., Yuzuriha, T., and Katayama, K. (1982).Biochim. Biophys. Acta 679, 308–314.

    Google Scholar 

  • Tsai, A. L., Kauten, R., and Palmer, G. (1985).Anal. Biochem. 151, 131–136.

    Google Scholar 

  • Van Blitterswijk, W. J., Van Hoeven, R. P., and Van Der Meer, B. W. (1981).Biochim. Biophys. Acta 644, 323–330.

    Google Scholar 

  • Vanella, A., Gorini, A., Campisi, A., and Villa, R. F. (1988).Gerontology 33, 271.

    Google Scholar 

  • Vanella, A., Villa, R. F., Gorini, A., Campisi, A., and Giuffrida-Stella, A. M. (1989).J. Neurosci. Res. 22, 351–355.

    Google Scholar 

  • Vanneste, W. H. (1966).Biochim. Biophys. Acta 113, 175–178.

    Google Scholar 

  • Villa, R. F. (1981).Eur. Neurol. 20, 245–252.

    Google Scholar 

  • Villa, R. F., Strada, P., Dagani, F., and Benzi, G. (1978).Biochem. Pharmacol. 27, 2278–2280.

    Google Scholar 

  • Villa, R. F., Benzi, G., and Curti, D. (1981).Biochem. Pharmacol. 30, 2398–2408.

    Google Scholar 

  • Villa, R. F., Marzatico, F., and Benzi, G. (1982a).Neurochem. Res. 8, 269–290.

    Google Scholar 

  • Villa, R. F., Curti, D., Polgatti, M., and Benzi, G. (1982b).J. Neurosci. Res. 7, 341–347.

    Google Scholar 

  • Villa, R. F., Gorini, A., Zanada, F., and Benzi, G. (1986).Arch. Int. Pharmacodyn. Ther. 279, 195–211.

    Google Scholar 

  • Villa, R. F., Gorini, A., Lo Faro, A., and Dall'Orbo, C. (1989a).Cell. Mol. Neurobiol. 9, 247–252.

    Google Scholar 

  • Villa, R. F., Gorini, A., Arnaboldi, R., Lo Faro, A., and Dall'Orbo, C. (1989b).Mech. Aging Dev. 49, 211–225.

    Google Scholar 

  • Villa, R. F., Arnaboldi, R., Gorini, A., and Geroldi, D. (1989c).Farm. Ed. Sci. 44, 215–226.

    Google Scholar 

  • Wilson, J. E. (1972).Arch. Biochem Biophys. 150, 96–104.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Biochimica, Università di Ancona, Italy

    Maurizio Battino & Enrico Bertoli

  2. Dipartimento di Biochimica, Università di Bologna, Italy

    Gabriella Formiggini & Simonetta Sassi

  3. Istituto di Farmacologia, Università di Pavia, Italy

    Antonella Gorini & Roberto F. Villa

  4. Dipartimento di Biologia Ev. Sper., Università di Bologna, Italy

    Giorgio Lenaz

Authors
  1. Maurizio Battino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Enrico Bertoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gabriella Formiggini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Simonetta Sassi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Antonella Gorini
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Roberto F. Villa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Giorgio Lenaz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Battino, M., Bertoli, E., Formiggini, G. et al. Structural and functional aspects of the respiratory chain of synaptic and nonsynaptic mitochondria derived from selected brain regions. J Bioenerg Biomembr 23, 345–363 (1991). https://doi.org/10.1007/BF00762227

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00762227

Key Words

Search

Navigation