Conclusions
-
1.
The formation of penicillin-like substances and natural penicillins during the biosynthesis of benzylpenicillin depends on the precursor concentration. The contents of the penicillin-like substances does not exceed 300–500 units/ml in the presence of PAM or PAA in the cultured liquid in quantities exceeding 0.05%.
-
2.
It was established that the consumption of the precursor by the fungus under various conditions (flasks and fermenters) is accomplished in different ways. In the flasks,a large portion of the precursor is incorporated into the penicillin molecule, whereas in the fermenters, a considerable amount of it is oxidized.
-
3.
The optimal precursor concentration and the method of its addition under laboratory conditions and in experimental fermenters were worked out.
-
4.
ThePenicillium chrysogenum 194 culture of various ages deaminate PAM differently. The deaminating ability increases with the age of the fungus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
M. M. Levitov, L. M. Lur'e, and G. F. Zavileiskaya, Antiobiotiki, No. 12, 1058 (1961).
L. Niiri and Z. L. Lengel', Ibid., No. 5, 396 (1965).
L. M. Lur'e and M. M. Levitov, Ibid., No. 5, 395 (1967).
S. C. Pan and D. Perlman, Anal. Chem.,26, 1432 (1954).
A. J. Moyer and R. D. Coghill, J. Bact.,53, 329 (1947).
K. Singh and M. Johnson, Ibid.,56, 339 (1948).
F. G. Jarvis and M. J. Johnson, Ibid.,59, 51 (1950).
Author information
Authors and Affiliations
Additional information
Translated from Khimiko-Farmatsevticheskii Zhurnal, No. 1, pp.27–31, January, 1969.
Rights and permissions
About this article
Cite this article
Lur'e, L.M., Verkhovtseva, T.P. & Levitov, M.M. The effect of the precursors on the formation of penicillin-like substances in the biosynthesis of benzylpenicillin. Pharm Chem J 3, 24–27 (1969). https://doi.org/10.1007/BF00759769
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00759769