Abstract
A cell kit named PREDISAFE based on the use of confluent rabbit fibroblastic cells has been designed to predict eye irritancy of cosmetic raw materials and formulations. The kit can be stored for a few days and/or shipped at room temperature. Cytotoxicity was estimated after 1 min or 15 min contact with test compounds using the neutral red release assay. For the 84 products tested, IC50 values gave intervals similar to classes defined from the Draize test, i.e., mild, moderate, severe and extreme irritancy.
Similar content being viewed by others
Abbreviations
- IC50 :
-
inhibiting concentration 50%
References
Borenfreund E, Puerner JA. A simple quantitative procedure using monolayer cultures for cytotoxicity assays. J Tissue Cult Methods. 1984;9:7–9.
Bruner LH, Kain DJ, Roberts DA, Parker RD. Evaluation of seven in vitro alternatives for ocular safety testing. Fundam Appl Toxicol 1991;17:136–49.
Draize JH, Woodard G, Calvery HO. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J Pharmacol Exp Ther. 1944; 82:377–90.
Grascha P, Chesne C, Peerally S. Etude in vitro de l'influence de l'association de tensioactifs anionique et amphotère sur la tolérance cutanée et oculaire. Rev Méd Trav. 1992;5:266–8.
Reader SJ, Blackwell V, O'Hara R, Clothier RH, Griffin G, Balls M. Neutral red release from pre-loaded cells as an in vitro approach to testing for eye irritancy potential. Toxicol In Vitro. 1990;4:264–6.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guyomard, C., Bouffechoux, J., Bourniche, J. et al. Evaluation of PREDISAFE, a cell kit for predicting eye irritancy of cosmetic raw materials and formulations. Cell Biol Toxicol 10, 375–379 (1994). https://doi.org/10.1007/BF00755785
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00755785