Cytotechnology

, Volume 16, Issue 2, pp 109–120 | Cite as

Identification of media supplements that improve the viability of primarily cell cultures ofCrassostrea gigas oysters

  • Isabelle Domart-Coulon
  • Dominique Doumenc
  • Stephanie Auzoux-Bordenave
  • Yann Le Fichant
Article

Abstract

Media supplements have been investigated for their influence on the viability of primary cell cultures from the heart ofCrassostrea gigas oysters. Soluble factors of vertebrate origin were tested, belonging to five families of supplements that had proven to increase the viability of insect and mammal cell cultures. Using two-level complete factorial assays, factors and mutual interactions were screened within each family with a MTT reduction assay. Results pointed out the positive influence of hormones, growth factor, antioxidants and lipids on the mitochondrial metabolism of oyter's heart cells. Consequently, a new concentrated complex supplement was developed. At 10% (v/v) final concentration in modified Leibovitz L-15 medium, it increases by 30% the cellular viability of one-week old cultures as compared with non-supplemented medium, a similar improvement as the one obtained with 10% (v/v) fetal calf serum. Combined with fetal calf serum, this new supplement doubles the cellular viability of one-week old cultures and allows networks of cardiomuscular cells to be maintained functional over three monthsin vitro.

Key words

Cell culture media development MTT Bivalvia oyster 

Abbreviations

MTT-3

(4,5-dimethylthiazol;-2-yl)2,5-diphenyl-tetrazolium bromide

FCS

fetal calf serum

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Isabelle Domart-Coulon
    • 1
  • Dominique Doumenc
    • 1
  • Stephanie Auzoux-Bordenave
    • 1
  • Yann Le Fichant
    • 2
  1. 1.Laboratoire de Biologie des Invertébrés Marins CNRS URA 699Muséum National d'Histoire NaturelleParisFrance
  2. 2.Bertin & CiePlaisir CedexFrance

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