Journal of Bioenergetics and Biomembranes

, Volume 15, Issue 6, pp 363–377 | Cite as

The adaptation to salinity: Response of fish gill mitochondria to salinity stress

  • N. Suresh
  • J. Jayaraman
Research Articles


When a freshwater fish,Tilapia mossambica (now renamedSarotherodon mossambicus) is exposed to a salinity stress, extensive changes are noted in the properties of the mitochondria isolated from the gill tissue. Our efforts were directed toward ascertaining the response of the gill mitochondria to an ion-osmotic shock to which the organism is exposed. Notable among them are the loss of ability to phosphorylate externally added ADP, decreased45Ca++ uptake, lower transhydrogenase levels, and nonresponse to ATP + Mg++ for contraction. During this period there is a large influx of calcium into the mitochondria. Continued exposure to the stress situation reverses virtually all the changes to the freshwater levels.

Key Words

Gill mitochondria salinity adaptation calcium uptake 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ambudkar, S. V., Khan, S. L., and Jayaraman, J. (1980). Phospholipid metabolism inE. coli during growth in high glucose medium,Indian J Biochem. Biophys. 17 361–365.Google Scholar
  2. Andreoli, T. E., Pharo, R. L., and Sanadi, D. R. (1964). On the mechanism of oxidative phosphorylation: further evidence for transhydrogenase reaction in submitochondrial particles,Biochim. Biophys. Acta 90 16–23.Google Scholar
  3. Bashyam, I., Suresh, N., and Jayaraman, J. (1980). Adaptation to salinity by fish: alterations in energy transducing status of muscle mitochondria,J. Biosci. 2 87–98.Google Scholar
  4. Brierley, G. P. (1976). The uptake and extrusion of monovalent cations by isolated heart mitochondria,Mol. Cell. Biochem. 10 41–62.Google Scholar
  5. Green, D. E., Mil, S., and Kohout, P. M. (1955). Studies on the terminal electron transport system,J. Biol. Chem. 217 551–567.Google Scholar
  6. Honnappa, G. V., Sulochana, H., and Jayaraman, J. (1975). Respiratory and calcium accumulating properties of muscle mitochondria of some aquatic arthropods,J. Bioenerg. 7 149–159.Google Scholar
  7. Kaplan, N. O. (1967).Methods Enzymol. 10 317.Google Scholar
  8. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with folin phenol reagent,J. Biol. Chem. 193 265–275.Google Scholar
  9. Loyter, A., Christiansen, R. O., Steensland, H., Saltzgager, J., and Racker, E. (1969). Energy-linked ion translocation in submitochondrial particles,J. Biol. Chem. 244 4422–4427.Google Scholar
  10. Mackler, B. (1967).Methods Enzymol. 10 261.Google Scholar
  11. Meenakshi, S., Rajamanickam, C., and Jayaraman, J. (1979). Adaptation to salinity by fish: macromolecular changes in mitochondria and microsomes of the gill,J. Biosci. 1 427–432.Google Scholar
  12. Sulochana, H. R., Bashyam, I., Suresh, N., and Jayaraman, J. (1977). Physiological correlates of calcium-accumulating properties of mitochondria: fish muscle mitochondria,J. Bioenerg. Biomembr. 9 337–348.Google Scholar
  13. Tzagoloff, A. (1970). Assembly of the mitochondrial membrane system,J. Biol. Chem. 245 1545–1551.Google Scholar
  14. Villalobo, A., and Lehninger, A. L. (1980). Inhibition of oxidative phosphorylation in ascites tumor mitochondria and cells by intramitochondrial calcium,J. Biol. Chem. 255 2457–2564.Google Scholar
  15. Warton, D. C., and Tzagoloff, A. (1967).Methods Enzymol. 10 245.Google Scholar

Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • N. Suresh
    • 1
  • J. Jayaraman
    • 1
  1. 1.Department of Biochemistry, School of Biological SciencesMadura Kamraj UniversityMaduraiIndia

Personalised recommendations