Summary
The Southern blot hybridization technique has been applied to study the configuration of immunoglobulin and T-cell receptor genes in 6 cases of the so called mediastinal large cell lymphoma with sclerosis. This lymphoma has been recently recognized as a separate entity among non-Hodgkin lymphomas mainly affecting young adult patients. The B-cell origin of this neoplasm was suggested by means of immunohistochemical analysis. However, the immunophenotypical B-cell related markers used do not always exhibit lineage fidelity. The Southern blot analysis demonstrated the presence of unique heavy and k-light chain immunoglobulin gene rearrangements, establishing genotypically their B-cell origin.
Similar content being viewed by others
References
Addis BJ, Isaacson PG (1986) Large cell lymphoma of the mediastinum: a B cell tumour of probable thymic origin. Histopathology 10:379–390
Arnold A, Cossman J, Bakhshi A, Jaffe ES, Waldmann TA, Korsmeyer SJ (1983) Immunoglobulin-gene rearrangements as unique clonal markers in human lymphoid neoplasms. N Engl J Med 309:1593–1599
Cleary ML, Chao J, Warnke R, Sklar J (1984) Immunoglobulin gene rearrangement as a diagnostic criterion of B-cell lymphoma. Proc Natl Acad Sci USA 81:593–597
Flug F, Pelicci PG, Bonetti F, Knowles DM II, Dalla Favera R (1985) T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms. Proc Natl Acad Sci USA 82:3460–3464
Griesser H, Feller A, Lennert K, Minden M, Mak TW (1986) Rearrangement of theβ chain of the T cell antigen receptor and immunoglobulin genes in lymphoproliferative disorders. J Clin Invest 78:1179–1184
Ha K, Minden M, Hozumi N, Gelfand EW (1984) Immunoglobulin gene rearrangement in acute myelogenous leukemia. Cancer Res 44:4658–4660
Hieter PA, Max EE, Seidman JG, Maizel JF, Leder P (1980) Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell 22:197–207
Isaacson PG, Spencer J, Connolly CE, Pollock DJ, Stein H, O'Connor NTJ, Bevan DH, Kirkham N, Wainscoat JS, Mason DY (1985) Malignant histiocytosis of the intestine: a T-cell lymphoma. Lancet iii:688–691
Knowles DM II, Dodson L, Burke JS, Wang M, Bonetti F, Pelicci PG, Flug F, Dalla Favera R, Wang CY (1985) Sig-E-(“Null Cell”) Non-Hodgkin's lymphomas: multiparametric determination of their B or T cell lineage. Am J Pathol 120:356–370
Korsmeyer SJ, Arnold A, Bakhshi A, Ravetch JV, Siebenlist U, Hieter PA, Sharrow SO, LeBien TW, Kersey JH, Pop-lack DG, Leder P, Waldmann TA (1983) Immunoglobulin gene rearrangement and cell surface antigen expression in acute lymphocytic leukemias of T cell and B cell precursor origins. J Clin Invest 71:301–313
Levine GD, Rosai J (1978) Thymic hyperplasia and neoplasia: a review of current concepts. Hum Pathol 9:495–515
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
Menestrina F, Chilosi M, Bonetti F, Lestani M, Scarpa A, Novelli P, Doglioni C, Todeschini G, Ambrosetti A, Fiore-Donati L (1986) Mediastinal large-cell lymphoma of B-type, with sclerosis: Histopathologic and immunohistochemical study of 8 cases. Histopathology 10:589–600
Möller P, Lämmler B, Eberlein-Gonska M, Feichter GE, Hoffman WJ, Schmitteckert H, Otto HF (1986) Primary mediastinal clear cell lymphoma of B-cell type. Virchows Arch A 409:79–92
O'Connor NTJ, Gatter KC, Wainscoat JS, Crick J, Jones DB, Delsol G, Ralfkiaer E, De Wolf-Peeters C, Angus B, Mason DY (1987) Practical value of genotypic analysis for diagnosing lymphoproliferative disorders. J Clin Pathol 40:147–150
Pelicci PG, Knowles DM, Dalla Favera R (1985) Lymphoid tumors displaying rearrangements of both immunoglobulin and T cell receptor genes. J Exp Med 162:1015–1024
Ravetch JV, Siebenlist U, Korsmeyer SJ, Waldmann TA, Leder P (1981) The structure of the human immunoglobulin mulocus: characterization of embryonic and rearranged J and D genes. Cell 27:583–591
Rigby PJ, Dieckman M, Rhodes C, Berg Q (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polimerase I. J Mol Biol 113:237–251
Rovigatti U, Mirro J, Kitchingman G, Dahl G, Ochs J, Murphy S, Stass S (1984) Heavy chain immunoglobulin gene rearrangement in acute nonlymphocytic leukemia. Blood 63:1023–1027
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Weiss LM, Trela BS, Cleary ML, Turner RR, Warnke RA, Sklar J (1985) Frequent immunoglobulin and T-cell receptor gene rearrangements in “hystiocytic” neoplasms. Am J Pathol 121:369–373
Yoshikai Y, Anatoniou D, Clark SP, Yanagi Y, Sangster R, Van den Elsen P, Terhorst C, Mak TW (1984) Sequence and expression of transcripts of the human T-cell receptorβ-chain genes. Nature 312:521–524
Yousem SA, Weiss LM, Warnke RA (1985) Primary mediastinal non-Hodgkin's lymphomas: A morphological and immunologic study of 19 cases. Am J Clin Pathol 83:676–680
Author information
Authors and Affiliations
Additional information
This work was supported by the Associazione Italiana per la Ricerca sul Cancro, Milano, Italy, and Progetto finalizzato Oncologia (contratto no 86.00461.44), CNR, Rome, Italy. Aldo Scarpa and Maurizio Lestani are supported by a Scholarship from the Associazione Italiana per la Ricerca sul Cancro, Milano, Italy
Rights and permissions
About this article
Cite this article
Scarpa, A., Bonetti, F., Menestrina, F. et al. Mediastinal large-cell lymphoma with sclerosis. Vichows Archiv A Pathol Anat 412, 17–21 (1987). https://doi.org/10.1007/BF00750725
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00750725