Chronic serum sickness glomerulonephritis: Passive immunisation inhibits the removal of glomerular antigen and electron dense deposits
- 29 Downloads
Chronic serum sickness glomerulonephritis was induced in 20 Wistar rats, using radio-labelled, chemically cationised bovine serum albumin (BSA) as antigen. Four days after the last injection of antigen, when relocation of antigen within the rat had effectively ceased, the rats were given a single large intraperitoneal dose of either non-immune rat gamma globulin or anti-BSA rat gamma globulin. Ten days later the rats were killed. The rats which had received the anti-BSA globulin had significantly more antigen in renal cortex and in isolated glomeruli than the control group. They also had larger mesangial deposits as assessed by morphometry at electron microscope level; assessment of subepithelial deposits provided equivocal results. These findings provide direct confirmation that circulating antibody which is directed against an antigen which is trapped within deposits in the glomerulus will inhibit the removal of the antigen and deposits from the mesangium.
Key wordsGlomerulonephritis Chronic serum sickness Immune complexes Passive immunisation
Unable to display preview. Download preview PDF.
- Agodoa YC, Mannik M (1987) Removal of subepithelial immune complexes with excess unaltered or cationic antigen. Lab Invest 32:13–18Google Scholar
- Furness PN, Turner DR (1987a) An assessment of the influence of antigen dose in two new models of chronic serum sickness glomerulonephritis in the rat. Br J Exp Path 68:527–538Google Scholar
- Furness PN, Turner DR (1988) Chronic serum sickness glomerulonephritis; antigen and deposit removal is inhibited by antibody but unaffected by glomerular phagocytes. J Pathol 155:354AGoogle Scholar
- Furness PN, Turner DR (1988) Chronic serum sickness glomerulonephritis: Modification of the immune response influences the rate of removal of mesangial electron dense deposits. J Pathol 156 (in press)Google Scholar
- Goode NP, Davison AM, Gowland G, Aparicio SR, Shires M (1985) Uptake and disposal of BSA-coated latex particles by the rat mesangium: Reaction with subsequently administered heterologous antiserum. J Pathol 147:189–198Google Scholar
- Haakenstad AO, Striker GE, Mannik M (1983) Removal of glomerular immune complex deposits by excess antigen in a chronic mouse model of immune complex disease. Lab Invest 48:323–331Google Scholar
- Hoare DG, Koshland DE (1967) A method for the quantitative modification and estimation of carboxylic acid groups in proteins. J Biol Chem 242:2447–2453Google Scholar
- Latta H, Fligiel S (1985) Mesangial fenestrations, sieving, filtration and flow. Lab Invest 52:591–598Google Scholar
- Mannik M, Striker GE (1980) Removal of glomerular deposits of immune complexes in mice by administration of excess antigen. Lab Invest 42:483–489Google Scholar