Summary
Two cases of Ki-1 lymphomas in childhood were analyzed immunohistochemically and immunoelectron microscopically. They expressed Hodgkin's disease associated antigen, Ki-1, interleukin-2 receptor (IL2R), OKT9, and HLA-DR. Histologically, the tumour cells were large in size with abundant cytoplasm and atypical nuclei. Lymph node involvement was characterized by parafollicular and marginal sinus infiltration. These features were identical to those reported in Ki-1 lymphomas. Electron microscopically tumour cells had abundant cytoplasmic organelles with pleomorphic nuclei but had no specific granules. Some tumour cells had marked interdigitation of cell membrane. Immunoelectron microscopically Ki-1 was positive on cell membrane. Tumour cells had no T-cell or B-cell antigens except for Leu-3 (T4). Unexpectedly they expressed epithelial membrane antigen (EMA) strongly. EMA was positive on cell membrane and in the cytoplasm. EMA was detected effectively in paraffin-embedded sections. Among the malignant lymphomas in childhood tested, two cases were EMA-positive. The pattern of EMA-reactivity and the histology were very similar to Ki-1 lymphomas. These results strongly suggest that Ki-1 lymphomas in childhood may arise from non-lymphoid haematopoietic cells and that EMA can be used as a new marker to distingish certain type of Ki-1 lymphomas in childhood.
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References
Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, Mason DY (1985) Production of monoclonal antibodies against human epithelial membrane angigen for use in diagnostic immunocytochemistry. Br J Cancer 52:347–354
Delsol G, Gatter KC, Stein H, Erber WN, Pulford KAF, Zinne K, Mason DY (1984) Human lymphoid cells express epithelial membrane antigen. Lancet ii:1124–1129
Gonzalez-Crussi F, Mangkornkanok M, Hsueh W (1987) Large-cell lymphoma. Diagnostic difficulties and case study. Am J Surg Pathol 11:59–65
Hata J, Ueyama Y, Tamaoki Y, Akatsuka A, Yoshimura S, Shimizu K, Morikawa Y, Furukawa T (1980) Human yolk sac tumor serially transplanted in nude mice: its morphologic and functional properties. Cancer 46:387–395
Hsu S, Yang K, Jaffe E (1985) Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease. Am J Pathol 118:209–217
Ishii E, Fujimoto J, Tanaka S, Hata J (1987) Immunohistochemical analysis on normal nephrogenesis and Wilms' tumour using monoclonal antibodies reactive with lymphohaematopoietic antigens. Virchows Arch A 411:315–322
Ishii Y, Kon S, Takei T, Fujimoto J, Kikuchi K (1983) Four distinct antigen system on human thymus and T cells defined by monoclonal antibodies: immunohistological and immunochemical studies. Clin Exp Immunol 53:31–40
Ishii Y, Takami T, Yuasa H, Takei T, Kikuchi K (1984) Two distinct antigen systems in human B lymphocytes: identification of cell surface and intracellular antigens using monoclonal antibodies. Clin Exp Immunol 58:183–192
Kadin ME, Sako D, Berliner N, Franklin W, Woda B, Borowitz M, Ireland K, Schweid A, Herzog P, Lange B, Dorfman R (1986) Childhood Ki-1 lymphoma presenting with skin lesions and peripheral lymphadenopathy. Blood 68:1042–1049
Kojima M, Imai Y (1973) Genesis and function of germinal center. Gann Monograph on Cancer Research 15:1–24
Rabkin MS, Kjeldsberg CR (1987) Epithelial membrane antigen staining patterns of histiocytic lesions. Arch Pathol Lab Med 111:337–338
Al Saati T, Caveriviere P, Gorguet B, Delson G, Garter KC, Mason DY (1986) Epithelial membrane antigen in hematopoietic neoplasms. Human Pathol 17:533–534
Schwab U, Stein H, Gerdes J, Lemke H, Kirchner H, Schaadt M, Diehl V (1982) Production of a monoclonal antibody specific for Hodgkin and Sternberg-Reed cells of Hodgkin's disease and a subset of normal lymphoid cells. Nature 299:65–67
Sloane JP, Ormerod MG (1981) Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer 47:1786–1795
Stein H, Mason DY, Gerdes J, O'Connor N, Wainscoat J, Pallesen G, Garter K, Falini B, Delsol G, Lemke H, Schwarting R, Lennert K (1985) The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood 66:848–858
Stewart SJ, Fujimoto J, Levy R (1986) Human T lymphocytes and monocytes bear the same Leu-3(T4) antigen. J Immunol 136:3773–3778
Takei T, Ishii Y, Kon S, Fujimoto J, Kikuchi K (1985) Determinant heterogeneity of CD5, CD8, and CD4 antigen molecules as defined by monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID (ed) Leukocyte typing II, Springer Verlag, New York, p 243–255
The non-Hodgkin's lymphoma pathologic classification project (1982) National Cancer Institute sponsored study of classifications of non-Hodgkin's lymphomas. Cancer 49:2112–2135
Wood GS, Warner NL, Warnke R (1983) Anti-Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol 131:212–215
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Fujimoto, J., Hata, Ji., Ishii, E. et al. Ki-1 lymphomas in childhood: Immunohistochemical analysis and the significance of epithelial membrane antigen (EMA) as a new marker. Vichows Archiv A Pathol Anat 412, 307–314 (1988). https://doi.org/10.1007/BF00750256
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DOI: https://doi.org/10.1007/BF00750256