Abstract
The addition of ethanol extracts ofCentella asiatica showed a remarkable enhancement of fibroblast cells attachment to Cytodex beads in serum-free (SF) medium. It also improves tPA production in both batch and perfusion cultivations. The optimal concentration for SF medium was determined as 2 ppm of the extracts when using Cytodex III. In batch cultivation a high specific tPA production rate was obtained, compared to that from 5% FBS containing medium. However, a fast specific growth rate was observed in 5% FBS medium. In perfusion cultivation a reasonably good cell density and tPA production was achieved at a perfusion rate of 2.4×106 (viable cell/ml) and 0.65 (μg/ml), respectively at 22 ml/min.
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Kim, Y.N., Park, Y.S., Kim, H.K. et al. Enhancement of the attachment on microcarriers and tPA production by fibroblast cells in a serum-free medium by the addition of the extracts ofCentella asiatica . Cytotechnology 13, 221–226 (1993). https://doi.org/10.1007/BF00749818
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DOI: https://doi.org/10.1007/BF00749818