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Production of monoclonal antibodies and ELISA for thebaine and codeine

Cytotechnology volume 19, pages 55–61 (1995)Cite this article

Abstract

The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Yukihiro Shoyama & Tomohiro Fukada

  2. Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-I Hakozaki, Higashi-ku, 812, Fukuoka, Japan

    Hiroki Murakami

Authors
  1. Yukihiro Shoyama
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  2. Tomohiro Fukada
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  3. Hiroki Murakami
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Shoyama, Y., Fukada, T. & Murakami, H. Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechnology 19, 55–61 (1995). https://doi.org/10.1007/BF00749755

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  • DOI: https://doi.org/10.1007/BF00749755

Key words

  • codeine
  • ELISA
  • monoclonal antibody
  • opium alkaloid
  • qualitative analysis
  • thebaine