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Production of monoclonal antibodies and ELISA for thebaine and codeine

Abstract

The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.

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Shoyama, Y., Fukada, T. & Murakami, H. Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechnology 19, 55–61 (1995). https://doi.org/10.1007/BF00749755

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  • DOI: https://doi.org/10.1007/BF00749755

Key words

  • codeine
  • ELISA
  • monoclonal antibody
  • opium alkaloid
  • qualitative analysis
  • thebaine