, Volume 11, Issue 2, pp 121–131 | Cite as

Purification and characterization of interferon-like antiviral protein derived from flatfish (Paralichthys olivaceus) lymphocytes immortalized by oncogenes

  • Tadakazu Tamai
  • Sanetaka Shirahata
  • Nobuyuki Sato
  • Shoji Kimura
  • Michio Nonaka
  • Hiroki Murakami
Original Research Papers


Flatfish leukocytes were transfected with the expression plasmids of the v-myc, c-myc, c-fos, v-myb and c-Ha-ras oncogenes. Only cotransfection of c-Ha-ras with c-myc or c-fos resulted in complete immortalization of the cells. Interferon-like anti-viral protein was found in the cultured medium of the immortalized lymphocytes. The protein was purified by DEAE-Toyopearl 650 M ion exchange chromatography and WGA agarose affinity chromatography. The protein was a glycoprotein of about 16 kDa. The antiviral activity of the protein was trypsin-sensitive and was fairly stable at pH values from 4 to 8. The protein retained about 60% of the activity even at 60°C and showed a broad antiviral activity for various fish cells and viruses.

Key words

cytokines fish leukocytes immortalization fish interferon oncogenes 


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Tadakazu Tamai
    • 1
  • Sanetaka Shirahata
    • 2
  • Nobuyuki Sato
    • 1
  • Shoji Kimura
    • 1
  • Michio Nonaka
    • 1
  • Hiroki Murakami
    • 2
  1. 1.Taiyo Central R&D InstituteTaiyo Fishery Co. LtdTsukuba-shi, IbarakiJapan
  2. 2.Graduate School of Genetic Resources TechnologyKyushu UniversityFukuokaJapan

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