Abstract
Phenylenediamines have been studied and compared as to their effectiveness in stimulating photosynthetic electron flux in DBMIB-inhibited chloroplasts. It has been found that N-substituted as well as C-substitutedp-phenylenediamines accelerate the rate of ferricyanide reduction, a photosystem II photoreaction, under conditions where the radical cations of N-substitutedp-phenylenediamines are stable. TheP/e 2 ratios for these partial reactions are between 0.4 and 0.5; this is taken as evidence that N-substitutedp-phenylenediamines are reduced by the choloroplasts close to the outer surface. Both N- and C-substitutedp-phenylenediamines are capable of bypassing the site of DBMIB inhibition and restoring electron flow from water to methylviologen. N-substitutedp-phenylenediamines appear to be more effective even at high concentrations of DBMIB. TheP/e 2 ratios for these reactions are on the order of 0.75–1.0; this is taken as evidence that the bypass reaction for N-substitutedp-phenylenediamines occurs on the inside of the thylakoid membrane.
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Abbreviations
- DBMIB:
-
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (dibromothymoquinone)
- PD:
-
p-phenylenediamine
- TMPD:
-
N,N,N′,N′-tetramethyl-p-phenylenediamine
- DMPD:
-
N,N-dimethyl-p-phenylenediamine
- FeCN:
-
potassium ferricyanide
- MV:
-
methylviologen
- BSA:
-
bovine serum albumin (Cohn-Fract. V-Serva)
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Selman, B.R. Phenylenediamine restoration of photosynthetic electron flux in DBMIB-inhibited chloroplasts. J Bioenerg Biomembr 8, 143–156 (1976). https://doi.org/10.1007/BF00748960
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DOI: https://doi.org/10.1007/BF00748960