Cytotechnology

, Volume 11, Supplement 1, pp S15–S17 | Cite as

Effect of trapidil derivative AR 12456 on intracellular cholesterol homeostasis in human hepatoma cell line Hep G2

  • Corsini A 
  • Grignaffini P 
  • Beitz J 
  • Mest HJ 
  • Bellosta S 
  • Fumagalli R 
  • Paoletti R 
Session 1 Hepatic Cell Cultures Selected Papers
  • 28 Downloads

Abstract

The effect of trapidil derivative AR12456 on intracellular cholesterol metabolism was investigated in human hepatoma cell line HepG2. AR12456 enhanced the uptake and degradation of125I-LDL in a dose-dependent manner. The drug inhibited cholesterol synthesis and esterification without affecting cellular cholesterol content and bile acid synthesis; cholesterol efflux was slightly increased. These results show that the inhibition of cholesterol synthesis together with the enhanced expression of LDL receptors may partially explain the hypocholesterolemic activity of compound AR12456.

Keywords

Cholesterol Synthesis Cholesterol Efflux Cell Line HepG2 Human Hepatoma Cell Line Bile Acid Synthesis 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Corsini A 
    • 1
  • Grignaffini P 
    • 1
  • Beitz J 
    • 2
  • Mest HJ 
    • 2
  • Bellosta S 
    • 1
  • Fumagalli R 
    • 1
  • Paoletti R 
    • 1
  1. 1.Institute of Pharmacological SciencesUniversity of MilanMilanItaly
  2. 2.Department of Pharmacology and ToxicologyMartin Luther University Halle-WittembergHalleGermany

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