Theoretical and Applied Genetics

, Volume 57, Issue 2, pp 89–95 | Cite as

Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an interspecificHordeum hybrid

  • T. J. Orton
  • R. P. Steidl


Hybrids ofHordeum vulgare (HV) xH. jubatum (HJ) were synthesized for purposes of introgressive breeding, but were sterile and the recovery of pure diploid tillers by colchicine applications in vivo was difficult. Plant regeneration from colchicine-treated callus cultures of the hybrid (HV x HJ) was investigated as a means to produce high numbers of pure diploid, fertile intermediates. 10 of 50 plants regenerated in this manner exhibited variable chromosome numbers with means of approximately 37 (expected diploid number = 42). Cytological examinations of microsporogenesis in all such plants revealed a high incidence of bivalent formation at metaphase I (as compared to nearly complete asynapsis in the F1), but spindle and chromosome abnormalities in later meiotic stages led to complete sterility. Approximately 40% of HJ plants regenerated from colchicine-treated calli appeared to be pure tetraploids of high fertility. These techniques are hence useful for high frequency production of diploid or polyploid plants.

Key words

Cytogenetics Plant regeneration Introgressive breeding Callus culture Regenerates Barley Hordeum hybrids 


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • T. J. Orton
    • 1
    • 2
  • R. P. Steidl
    • 1
    • 3
  1. 1.Department of Crop and Soil Sciences MichiganState UniversityEast LamingUSA
  2. 2.Department of Vegetable CropsUniversity of CaliforniaDavisUSA
  3. 3.Cargill SeedsMonticelloUSA

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