Antibody block of a neural-tissue-specific glycoconjugate perturbs growth cone guidance of an identified interneuron in the grasshopper
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Antibodies against horseradish peroxidase (HRP) recognize a neural-tissue-specific carbohydrate moiety that is expressed on a complex set of developmentally regulated antigens in grasshopper,Drosophila and other insects. The functional role of the neural-specific carbohydrate has been investigated by mutant analysis inDrosophila where subtle defects in wing sensory axon projections have been reported. Here we extend the analysis of this neural-specific carbohydrate to the single cell level by focusing on identified brain interneurons in the grasshopper embryo. Immunological blocking experiments carried out in embryo culture show that the neural-specific carbohydrate is essential for correct axonal guidance of the identified interneurons. Functional block of the carbohydrate epitope causes major aberrations in growth cone guidance and axonal outgrowth in approximately 40% of the cases studied. This analysis reveals an important role of neural-specific glycoconjugate for correct axonal guidance of individual identified neurons.
Key wordsAxogenesis Anti-HRP Process outgrowth Axonal guidance TERM-1
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