Roux's archives of developmental biology

, Volume 204, Issue 1, pp 75–78 | Cite as

Antibody block of a neural-tissue-specific glycoconjugate perturbs growth cone guidance of an identified interneuron in the grasshopper

  • Fukang Xie
  • Thomas Meier
  • Heinrich Reichert
Short Communication

Abstract

Antibodies against horseradish peroxidase (HRP) recognize a neural-tissue-specific carbohydrate moiety that is expressed on a complex set of developmentally regulated antigens in grasshopper,Drosophila and other insects. The functional role of the neural-specific carbohydrate has been investigated by mutant analysis inDrosophila where subtle defects in wing sensory axon projections have been reported. Here we extend the analysis of this neural-specific carbohydrate to the single cell level by focusing on identified brain interneurons in the grasshopper embryo. Immunological blocking experiments carried out in embryo culture show that the neural-specific carbohydrate is essential for correct axonal guidance of the identified interneurons. Functional block of the carbohydrate epitope causes major aberrations in growth cone guidance and axonal outgrowth in approximately 40% of the cases studied. This analysis reveals an important role of neural-specific glycoconjugate for correct axonal guidance of individual identified neurons.

Key words

Axogenesis Anti-HRP Process outgrowth Axonal guidance TERM-1 

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References

  1. Bentley D, Keshishian H, Shankland M, Toroian-Raymond A (1979) Quantitative staging of embryonic development of the grasshopper,Schistocerca nitens. J Embryol Exp Morphol 54: 47–74Google Scholar
  2. Caudy M, Bentley DJ (1986) Pioneering growth cone steering along a series of neuronal and non-neuronal cues of different affinities. J Neurosci 6:1781–1795Google Scholar
  3. Harlow E, Lane D (1988)Antibodies — a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbour, New YorkGoogle Scholar
  4. Jan LY, Jan YN (1982) Antibodies to horseradish peroxidase as specific neuronal markers inDrosophila and grasshopper embryos. Proc Natl Acad Sci USA 79:2700–2704Google Scholar
  5. Katz F, Moats W, Jan YN (1988) A carbohydrate epitope expressed uniquely on the cell surface ofDrosophila neurons is altered in the mutantnac (neurally altered carbohydrate). EMBO J 7:3471–3477Google Scholar
  6. Meier T, Therianos S, Zacharias D, Reichert H (1993) Developmental expression of TERM-1 glycoprotein on growth cones and terminal arbors of individual identified neurons in the grasshopper. J Neurosci 13:1498–1510Google Scholar
  7. Reichert H (1993) Molecular correlates of neuronal specificity in the developing insect nervous system. Mol Neurobiol 7:349–362Google Scholar
  8. Snow PM, Patel NH, Harrelson AL, Goodman CS (1987) Neural-specific carbohydrate shared by many surface glycoproteins inDrosophila and grasshopper embryos. J Neurosci 7:4137–4144Google Scholar
  9. Whitlock KE (1993) Development ofDrosophila wing sensory neurons in mutants with missing or modified cell surface molecules. Development 117:1251–1260Google Scholar
  10. Xie F, Meier T, Reichert H (1994) Coexpression and function of TERM-1 and fasciclin II during axonal outgrowth of identified brain interneurons in the grasshopper. Proc R Soc Lond B 257:199–204Google Scholar
  11. Zacharias D, Williams LJD, Meier T, Reichert H (1993) Neurogenesis in the insect brain: cellular identification and molecular characterization of brain neuroblasts in the grasshopper embryo. Development 118:941–955Google Scholar

Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • Fukang Xie
    • 1
  • Thomas Meier
    • 1
  • Heinrich Reichert
    • 1
  1. 1.Department of ZoologyUniversity of BaselBaselSwitzerland

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