Roux's archives of developmental biology

, Volume 204, Issue 1, pp 20–29 | Cite as

Mesoderm differentiation in explants of carp embryos

  • Valentina Bozhkova
  • Geertruy te Kronnie
  • Lucy P. M. Timmermans
Original Article


An analysis of carp blastoderm development was carried out in culture after isolation from the yolk cell and its yolk syncytial layer (YSL). The blastoderms were separated from the YSL at four different stages of embryogenesis: the blastula, early epiboly, early gastrula and late gastrula stages. Absence of the YSL in explants was checked by scanning electron microscopy. From observations of living embryos and histological examination of tissues which were formed in explants from all stages studied it was observed that they contained notochordal, muscle and neural tissue as signs of dorsal types of differentiation. Only in explants from the early and late gastrula stages were histotypical tissues organized in an embryonic-like body pattern. The data indicate that mesoderm differentiation in fish embryos is independent from the YSL, contrary to normal pattern formation which needs the presence of the YSL before the onset of gastrulation.

Key words

Fish development Cyprinus carpio Explants Mesoderm differentiation 


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Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • Valentina Bozhkova
    • 1
  • Geertruy te Kronnie
    • 2
  • Lucy P. M. Timmermans
    • 2
  1. 1.Institute for Information Transmission ProblemsRussian Academy of SciencesMoscowRussia
  2. 2.Department of Animal Morphology and Cell BiologyAgricultural UniversityWageningenThe Netherlands

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