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Roux's archives of developmental biology

, Volume 204, Issue 1, pp 20–29 | Cite as

Mesoderm differentiation in explants of carp embryos

  • Valentina Bozhkova
  • Geertruy te Kronnie
  • Lucy P. M. Timmermans
Original Article

Abstract

An analysis of carp blastoderm development was carried out in culture after isolation from the yolk cell and its yolk syncytial layer (YSL). The blastoderms were separated from the YSL at four different stages of embryogenesis: the blastula, early epiboly, early gastrula and late gastrula stages. Absence of the YSL in explants was checked by scanning electron microscopy. From observations of living embryos and histological examination of tissues which were formed in explants from all stages studied it was observed that they contained notochordal, muscle and neural tissue as signs of dorsal types of differentiation. Only in explants from the early and late gastrula stages were histotypical tissues organized in an embryonic-like body pattern. The data indicate that mesoderm differentiation in fish embryos is independent from the YSL, contrary to normal pattern formation which needs the presence of the YSL before the onset of gastrulation.

Key words

Fish development Cyprinus carpio Explants Mesoderm differentiation 

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References

  1. Ballard WW (1973) Origin of the hypoblast inSalmo. I. Does the blastodisc edge turn inward? J Exp Zool 161:201–210Google Scholar
  2. Bozhkova VP, Palmbakh LR, Khariton VYu, Chaylakhyan LM (1983) Organization of the surface and adhesive properties of cleavage furrows in loach (Misgurnus fossilis) eggs. Exp Cell Res 149:129–139Google Scholar
  3. Dale L, Slack J (1987) Fate map of the 32-cell stage ofXenopus laevis. Development 99:527–551Google Scholar
  4. Dale L, Howes G, Price BMJ, Smith JC (1992) Bone morphogenetic protein 4: a ventralizing factor in earlyXenopus development. Development 115:573–585Google Scholar
  5. David I (1992) Mesoderm induction and axis determination inXenopus laevis. Bio Essays 14:687–692Google Scholar
  6. Devillers C (1961) Structural and dynamic aspects of the development of the teleostean egg. Adv Morphog 1:379–424Google Scholar
  7. Fleig R (1990) Gastrulation in the zebrafishBrachydanio rerio (Teleostei) as seen in the scanning electron microscope. Experimental embryology in aquatic plants and animals. Marthy HJ (ed) Plenum Press, New York, pp 329–338Google Scholar
  8. Gallagher B, Hainski A, Moody S (1991) Autonomous differentiation of dorsal axial structures from an animal cap of cleavage blastomeres inXenopus. Development 112:1103–1114Google Scholar
  9. Gevers P, Timmermans LPM (1991) Dye-coupling and the formation and fate of the hypoblast in the teleost fish embryo,Barbus conchonius. Development 112:431–438Google Scholar
  10. Gevers P, Coenen AJ, Schipper H, Stroband HWJ, Timmermans LPM (1993) Involvement of fibronectin during epiboly and gastrulation in embryos of the common carp,Cyprinus carpio. Roux's Arch Dev Biol 202:152–158Google Scholar
  11. Helde KA, Grunwald DJ (1993) The DVR-1 (Vg1) transcript of zebrafish is maternally supplied and distributed throughout the embryo. Dev Biol 159:418–426Google Scholar
  12. Ho RK (1992) Cell movements and cell fate during zebrafish gastrulation. Development (Suppl):137–142Google Scholar
  13. Ho RK, Kimmel CB (1993) Commitment of cell fate in the early zebrafish embryo. Science 261:109–111Google Scholar
  14. Holtfreter J (1931) Über die Aufzucht isolierter Teile des Amphibienkeimes. II Züchtung von Keimen und Keimteilen in Salzlösung. Arch Entwicklungsmech Org 124:403–466Google Scholar
  15. Howard JE, Smith JC (1993) Analysis of gastrulation: different types of gastrulation movement are induced by different mesoderm-inducing factors inXenopus laevis. Mech Dev 43:37–48Google Scholar
  16. Jones CM, Lyons KM, Lapan PM, Wright CVE, Hogan BLM (1992) BVR-4 (bone morphogenetic protein-4) as a posteriorventralizing factor inXenopus mesoderm induction. Development 115:639–647Google Scholar
  17. Kane DA, Warga RM, Kimmel CB (1992) Mitotic domains in the early embryo of the zebrafish. Nature 360:735–737Google Scholar
  18. Keller R, Trinkaus J (1987) Rearrangement of enveloping layer cells without disruption of the epithelial permeability barrier as a factor inFundulus epiboly. Dev Biol 120:12–24Google Scholar
  19. Kimmel CB, Law R (1985) Cell lineage of zebrafish blastomeres. II. Formation of the yolk syncytial layer. Development 108: 86–93Google Scholar
  20. Kimmel CB, Spray DC, Bennett MVL (1984) Developmental uncoupling between blastoderm and yolk cell in the embryo of the teleostFundulus. Dev Biol 102:483–487Google Scholar
  21. Kimmel CB, Warga RM, Schilling TF (1990) Origin and organization of the zebrafish fate map. Development 108:581–594Google Scholar
  22. Kostomarova AA (1969) The differentiation capacity of isolated loach (Misgurnus fossilis) blastomeres. J Embryol Exp Morphol 22:407–430Google Scholar
  23. Long WL (1983) The role of the yolk syncytial layer in determination of the plane of bilateral symmetry in the rainbow trout,Salmo gairdneri, Richardson. J Exp Zool 228:91–97Google Scholar
  24. Nieuwkoop PD (1973) The “organization centre” of the amphibian embryo, its origin, spatial organization and morphogenetic action. Adv Morphol 10:1–39Google Scholar
  25. Oppenheimer JM (1936) The development of isolated blastoderms ofFundulus heteroclitus. J Exp Zool 72:247–269Google Scholar
  26. Oppenheimer JM (1938) Potencies for differentiation in the teleostean germ ring. J Exp Zool 2:185–212Google Scholar
  27. Rott NN, Bozhkova VP, Kvavilashvili ISh, Khariton VYu, Sharova LV (1978) Development of the isolated blastoderms of the loach (Misgurnus fossilis) upon cultivation in different saline media (in Russian). Sov J Dev Biol 9:457–468Google Scholar
  28. Schulte-Merker S, Ho RK, Herrmann BG, Nüsslein-Volhard C (1992) The protein product of zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo. Development 116:1021–1032Google Scholar
  29. Smith J (1989) Mesoderm induction and mesoderm-inducing factors in early amphibian development. Development 105: 665–677Google Scholar
  30. Stachel SE, Grunwald DJ, Myers PZ (1993) Lithium perturbation andgoosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish. Development 117:1261–1274Google Scholar
  31. Stroband H, te Kronnie G, van Gestel W (1992) Differential susceptibility of early steps in carp (Cyprinus carpio) development to α-amanitin. Roux's Arch Dev Biol 202:61–65Google Scholar
  32. Trinkaus JP (1984) Mechanism ofFundulus epiboly — a current view. Am Zool 24:673–688Google Scholar
  33. Trinkaus J, Drake JW (1956) Exogenous control of morphogenesis in isolatedFundulus blastoderms by nutrient chemical factors. J Exp Zool 132:311–342Google Scholar
  34. Tung T-C, Chang C-I, Tung I-E (1945) Experiments on the developmental potencies of eggs separated latitudinally. Proc Zool Soc Lond 115:175–188Google Scholar
  35. Warga RM, Kimmel CB (1990) Cell movements during epiboly and gastrulation in zebrafish. Development 108:569–580Google Scholar
  36. Whitman M, Melton DA (1992) Involvement of p21ras inXenopus mesoderm induction. Nature 357:252–255Google Scholar
  37. Winklbauer R (1990) Mesodermal cell migration duringXenopus gastrulation. Dev Biol 142:155–168Google Scholar
  38. Wood A, Timmermans LPM (1988) Teleost epiboly: a reassessment of deep cell movement in the germ ring. Development 102:575–585Google Scholar

Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • Valentina Bozhkova
    • 1
  • Geertruy te Kronnie
    • 2
  • Lucy P. M. Timmermans
    • 2
  1. 1.Institute for Information Transmission ProblemsRussian Academy of SciencesMoscowRussia
  2. 2.Department of Animal Morphology and Cell BiologyAgricultural UniversityWageningenThe Netherlands

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