Journal of Bioenergetics and Biomembranes

, Volume 17, Issue 4, pp 217–223 | Cite as

Structural and related functional changes in sarcoplasmic reticulum induced by long-chain fatty acids

  • F. M. Munkonge
  • C. D. Stubbs
  • P. J. Quinn
Research Articles


The effect of palmitic and oleic acids on Ca2+-ATPase activity in coupled preparations of sarcoplasmic reticulum isolated from rabbit hind leg muscle have been compared with their effects on vesicles uncoupled with Ca2+ ionophore, A23187. Palmitate at 2 µM · mg protein−1 has no significant effect on enzyme activity and does not uncouple catalytic activity from calcium accumulation within the vesicles. Oleic acid at 1 µM · mg protein−1 uncouples the vesicles, whereas 2 µM · mg protein−1 completely inhibits Ca2+-ATPase activity. Fluorescence anisotropy of diphenylhexatriene is not significantly altered by palmitate, but a large transient increase in motion of the probe is observed with addition of oleic acid. The effects of oleic acid on enzyme activity are not mediated via an effect on the bulk properties of the hydrophobic domain of the membrane lipids.

Key Words

Ca2+-ATPase sarcoplasmic reticulum calcium transport fatty acids membrane organization 


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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • F. M. Munkonge
    • 1
  • C. D. Stubbs
    • 2
  • P. J. Quinn
    • 1
  1. 1.Department of BiochemistryKing's College London (KQC)LondonU.K.
  2. 2.Department of Pathology and Laboratory MedicineHahnemann UniversityPhiladelphia

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