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The role of Mg2+ in the regulation of the structural and functional steady-states in rat liver mitochondria

Journal of Bioenergetics and Biomembranes volume 15, pages 217–234 (1983)Cite this article

Abstract

A possible relationship between mitochondrial Mg2+ levels, structural configurations, and functional steady states has been studied in rat liver mitochondria. The results show that the concentration of mitochondrial Mg2+ in respiratory state 4 is definitely higher than in respiratory state 3. The metabolic transition from state 3 to state 4 and vice-versa is associated with reversible influx-efflux of about 10 nmol of Mg2+ per mg protein. The net uptake of this aliquot of Mg2+ is a necessary condition in order for the metabolic transition to state 4, both structurally and functionally, to occur. This process requires a threshold concentration of external Mg2+ greater than 5 mM. The phosphorylative mechanism does not appear to depend on the presence or absence of external Mg2+. The role of Mg2+ on the attainment and maintenance of the structural and functional steady state 4 seems to be correlated with its regulatory effect on the concentration of the mitochondrial Pi.

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Affiliations

  1. Institutes of Biological Chemistry and General Pathology, University of Modena, 41 100, Modena, Italy

    Alberto Masini, Daniela Ceccarelli-Stanzani & Umberto Muscatello

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  1. Alberto Masini
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  2. Daniela Ceccarelli-Stanzani
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  3. Umberto Muscatello
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Masini, A., Ceccarelli-Stanzani, D. & Muscatello, U. The role of Mg2+ in the regulation of the structural and functional steady-states in rat liver mitochondria. J Bioenerg Biomembr 15, 217–234 (1983). https://doi.org/10.1007/BF00743942

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  • DOI: https://doi.org/10.1007/BF00743942

Key Words

  • Mg2+ levels
  • respiratory states
  • phosphorylative efficiency
  • rat liver mitochondria