Abstract
Addition to rat liver mitochondria of 2 mM inorganic phosphate or 0.15 mM diamide, a thiol-oxidizing agent, induced an efflux of endogenous Mg2+ linear with time and dependent on coupled respiration. No net Ca2+ release occurred under these conditions, while a concomitant release of K+ was observed. Mg2+ efflux mediated either by Pi or low concentrations of diamide was completely prevented by EGTA, Ruthenium red, and NEM. These reagents also inhibited the increased rate of state 4 respiration induced both by Pi and diamide. At higher concentrations (0.4 mM), diamide induced an efflux of Mg2+ which was associated also with a release of endogenous Ca2+. Under these conditions EGTA completely prevented Mg2+ and K+ effluxes, while they were only partially inhibited by Ruthenium red and NEM. It is assumed that Mg2+ efflux, occurring at low diamide concentrations or in the presence of phosphate, is dependent on a cyclic in-and-out movement of Ca2+ across the inner mitochondrial membrane, in which the passive efflux is compensated by a continuous energy linked reuptake. This explains the dependence of Mg2+ efflux on coupled respiration, as well as the increased rate of state 4 respiration. The dependence of Mg2+ efflux on phosphate transport is explained by the phosphate requirement for Ca2+ movement.
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Abbreviations
- Diamide:
-
diazenedicarboxylic acidbis-dimethylamide
- FCCP:
-
p-trifluoromethoxyphenylhydrazone
- EGTA:
-
ethylene glycol-bis-(2-amino ethyl ether)-N,N′-tetracetic acid
- Pi :
-
inorganic phosphate
- Ruthenium red:
-
Ru2(OH)2Cl4 · 7NH3 · 3H2O
- state 4:
-
controlled state of respiration in the presence of substrate
- RCI:
-
respiratory control index
- NEM:
-
N-ethyl maleimide
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A partial and preliminary report of these results has been published inBiochem. Biophys. Res. Comm.,78 (1977) 23.
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Siliprandi, D., Toninello, A., Zoccarato, F. et al. Efflux of magnesium and potassium ions from liver mitochondria induced by inorganic phosphate and by diamide. J Bioenerg Biomembr 10, 1–11 (1978). https://doi.org/10.1007/BF00743223
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DOI: https://doi.org/10.1007/BF00743223