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Light-induced generation of electric potential difference in membranes of purple and green sulfur bacteria

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Abstract

When associated with a planar phospholipid membrane, chromatophores isolated from photosynthetic sulfur bacteriaChromatium minutissimum, Ectothiorhodospira shaposhnikovii, andChlorobium limicola f. thiosulfatophilum were shown to generate a light-induced transmembrane electric potential difference measured by a direct method using macroelectrodes and a voltmeter. The maximal photoelectric responses were observed upon the addition of 1,4-naphthoquinone in combination with phenazine methosulfate (or TMPD) and ascorbate. The photoeffects were inhibited by CCCP and gramicidin. The data demonstrate that similar mechanisms of photoelectric generation function in membranes of the different bacteria studied.

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References

  1. L. A. Drachev, V. N. Frolov, A. D. Kaulen, A. A. Kondrashin, V. D. Samuilov, A. Yu. Semenov, and V. P. Skulachev,Biochim. Biophys. Acta 440 (1976) 637.

    Google Scholar 

  2. P. Mitchell,Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation, Glynn Research, Bodmin (1966).

    Google Scholar 

  3. L. A. Drachev, A. A. Kondrashin, V. D. Samuilov, and V. P. Skulachev,FEBS Lett. 50 (1975) 219.

    Google Scholar 

  4. E. A. Liberman and L. M. Tsofina,Biofizika 14 (1969) 6.

    Google Scholar 

  5. E. L. Barsky, E. A. Bonch-Osmolovskaya, S. A. Ostroumov, V. D. Samuilov, and V. P. Skulachev,Biochim. Biphys. Acta 375 (1975) 434.

    Google Scholar 

  6. E. L. Barsky and V. D. Samuilov,J. Bioenerg. 4 (1973) 391.

    Google Scholar 

  7. V. D. Samuilov and E. N. Kondratieva,Biol. Nauki 5 (1969) 97.

    Google Scholar 

  8. L. A. Drachev, A. A. Jasaitis, A. D. Kaulen, A. A. Kondrashin, E. A. Liberman, I. B. Nemecek, S. A. Ostroumov, A. Yu. Semenov, and V. P. Skulachev,Nature 249 (1974) 321.

    Google Scholar 

  9. J. A. Bergeron, in:Structure and Function of the Photosynthetic Apparatus, IL, Moscow (1962) p. 131.

    Google Scholar 

  10. N. E. Cherni, G. V. Solov'eva, V. D. Fedorov, and E. N. Kondratieva,Mikrobiologiya 38 (1969) 479.

    Google Scholar 

  11. C. O. L. Boyce, S. H. Oyewole, and R. C. Fuller, in:Abstracts of Brookhaven Symposium in Biology, No. 28 (1976) p. G1.

  12. E. N. Kondratieva,Photosynthezirujuschie Bakterii, Publ. Acad. Sci. of USSR, Moscow (1963).

    Google Scholar 

  13. A. Trebst,Z. Naturforsch. 31 (1976) 152.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Biological Faculty Moscow State University, 117234, Moscow, USSR

    N. P. Krasinskaya & V. D. Samuilov

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  1. N. P. Krasinskaya
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  2. V. D. Samuilov
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Krasinskaya, N.P., Samuilov, V.D. Light-induced generation of electric potential difference in membranes of purple and green sulfur bacteria. J Bioenerg Biomembr 9, 171–180 (1977). https://doi.org/10.1007/BF00743191

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  • DOI: https://doi.org/10.1007/BF00743191

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