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Influence of culture parameters on lignin metabolism byPhanerochaete chrysosporium

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Culture parameters influencing metabolism of synthetic14C-lignins to14CO2 in defined media have been studied in shallow batch cultures of the ligninolytic wood-destroying HymenomycetePhanerochaete chrysosporium Burds. Study of the effect of O2 concentration in the gas phase above non-agitated cultures indicated essentially complete absence of attack on the lignin polymer at 5% O2 in N2, and a 2- to 3-fold enhancement by 100% O2 as compared to air (21% O2). Agitation of the cultures resulting in the formation of mycelial pellets greatly suppressed lignin decomposition. The optimum culture pH for lignin decomposition was 4 to 4.5, with marked suppression above 5.5 and below 3.5. The source of nutrient nitrogen (NO 3 , NH +4 , amino acids) had little influence on lignin decomposition, but the concentration of nitrogen was critical; decomposition at 24 mM was only 25–35% of that at 2.4 mM N. Thiamine was the only vitamin required for growth and lignin decomposition. Under the optimum conditions developed, decomposition of 5 mg of synthetic lignin was accompanied by utilization of approximately 100 mg of glucose. The influence of the various culture parameters was analogous for metabolism of synthetic lignin labeled in the ring-,side chain-, and methoxyl carbon atoms.

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  • Bu'Lock, J. D., Detroy, R. W., Hostalek, Z., Munum-Al-Shakarchi, A.: Regulation of secondary biosynthesis inGibberella fujikuroi. Trans. Brit. Mycol. Soc.62, 377–389 (1974)

    Google Scholar 

  • Connors, W. J., Lorenz, L. F., Kirk, T. K.: Chromatographic separation of lignin models by molecular weight using Sephadex LH-20. Holzforschung32 (in press, 1978)

  • Gottlieb, S., Pelczar, M. J., Jr.: Microbiological aspects of lignin degradation. Bacteriol. Rev.15, 55–76 (1951)

    Google Scholar 

  • Hackett, W. F., Connors, W. J., Kirk, T. K., Zeikus, J. G.: Microbial decomposition of synthetic14C-labeled lignins in a variety of natural materials. Appl. Environ. Microbiol.33, 43–51 (1977)

    Google Scholar 

  • Kirk, T. K., Chang, H.-m.: Decomposition of lignin by white-rot fungi. II. Characterization of heavily degraded lignins from decayed spruce. Holzforschung29, 56–64 (1975)

    Google Scholar 

  • Kirk, T. K., Connors, W. J., Bleam, R. D., Hackett, W. F., Zeikus, J. G.: Preparation and microbial decomposition of synthetic [14C]-lignins. Proc. Nat. Acad. Sci. (Wash.)72, 2515–2519. (1975)

    Google Scholar 

  • Kirk, T. K., Connors, W. J., Zeikus, J. G.: Requirement for a growth substrate during lignin decomposition by two wood-rotting fungi. Appl. Environ. Microbiol.32, 192–194 (1976)

    Google Scholar 

  • Konishi, K., Inoue, Y.: Decomposition of lignin byCoriolus versicolor. III. Mode of action of laccase-type enzyme on lignin. J. Jap. Wood Res. Soc.17, 255–262 (1971)

    Google Scholar 

  • Lundquist, K., Kirk, T. K., Connors, W. J.: Fungal degradation of kraft lignin and lignin sulfonates prepared from synthetic14C-lignin. Arch. Microbiol.112, 291–296 (1977)

    Google Scholar 

  • Nelson, N.: A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem.153, 375–380 (1944)

    Google Scholar 

  • Phillips, D. H.: Oxygen transfer into myceial pellets. Biotech. Bioeng.8, 456–460 (1966)

    Google Scholar 

  • Saeman, J. F., Moore, W. E., Mitchell, R. L., Millett, M. A.: Techniques for the determination of pulp constituents by quantitative paper chromatography. Tappi37, 336–343 (1954)

    Google Scholar 

  • Tono, T., Tani, Y., Ono, K.: Microbial treatment of agricultural wastes. I. Adsorption of lignin and clarification of lignin-containing liquor by moulds. J. Ferment. Technol.46, 569–576 (1968)

    Google Scholar 

  • Vogel, A. I.: A textbook of practical organic chemistry. New York: Wiley 1956

    Google Scholar 

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Kirk, T.K., Schultz, E., Connors, W.J. et al. Influence of culture parameters on lignin metabolism byPhanerochaete chrysosporium . Arch. Microbiol. 117, 277–285 (1978).

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