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Glycoconjugate Journal

, Volume 10, Issue 2, pp 165–169 | Cite as

UDP-galactose:lactosylceramide α-galactosyltransferase activity in human placenta

  • Anja Lampio
  • Antero Airaksinen
  • Hannu Maaheimo
Papers

Abstract

The activity of UDP-Gal: LacCer galactosyltransferase in human placenta was studied by using crude homogenate and Triton CF-54 extract as the source of enzyme. Transfer of galactose to lactosylceramide was optimal in the presence of 0.1% Triton CF-54 and Mn2+ at pH 6.3, and the reaction product was susceptible to α-galactosidase.

Keywords

glycolipid biosynthesis p blood group globotriaosylceramide Pk antigen 

Abbreviations

LacCer

lactosylceramide (Galβ1-4Glcβ1-1Cer)

Gb3

globotriaosylceramide (Galα1-4Galβ1-4Glcβ1-1Cer)

Gb4

globoside (GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1Cer)

TLC

thin-layer chromatography

GC/MS

gas chromatography/mass spectrometry

NMR

nuclear magnetic resonance

EDTA

ethylenediamine tetraacetic acid

CHAPS

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Anja Lampio
    • 1
    • 2
  • Antero Airaksinen
    • 1
  • Hannu Maaheimo
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of HelsinkiHelsinkiFinland
  2. 2.Institute of BiotechnologyUniversity of HelsinkiHelsinkiFinland

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