Mucopolysaccharide histochemistry of rat tooth germs

  • Giuliano Quintarelli
  • Marie C. Dellovo


The histochemical distribution and localization of acid mucopolysaccharides and glycoproteins have been studied in rat tooth germs and lower joints. After fixation, sections were stained with PAS, alcian blue-PAS, colloidal iron, colloidal iron-PAS, colloidal iron-Feulgen, azure A and safranin O. Prior to staining, sections were incubated in hyaluronidase, pepsin and papain. A 2 hour incubation in pepsin greatly enhanced AB or CI basophilia in the stellate reticulum and the enamel-dentinal junction. These findings demonstrated that some acidic radicals of the polysaccharides are partially blocked by basic proteins. Hyaluronidase did not completely destroy the alcian blue or colloidal iron positive material whereas azure A metachromasia was completely removed. These results indicated that AB and/or CI stained substrates different from those revealed by azure A.


Iron Polysaccharide Acidic Radical Basic Protein Papain 
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Copyright information

© Springer-Verlag 1963

Authors and Affiliations

  • Giuliano Quintarelli
    • 1
    • 2
  • Marie C. Dellovo
    • 1
  1. 1.University of Alabama Medical CenterBirmingham
  2. 2.University of Reme Medical SchoolRomeItaly

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