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Some histochemically important properties of ribonuclease isolated from fixed tissues

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Zeitschrift für Zellforschung und mikroskopische Anatomie Abt. Histochemie Aims and scope Submit manuscript

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References

  • Anfinsen, C. R., R. R. Redfield, W. L. Choate, J. Page andW. R. Carrol: Studies on the gross structure, cross-linkages, and terminal sequences in ribonuclease. J. biol. Chem.207, 201–210 (1954).

    Google Scholar 

  • Brachet, J.: La détection histochimique des acides pentosenucleiques. C.R. Soc. Biol. (Paris)133, 88–89 (1940).

    Google Scholar 

  • —— Biochemical Cytology. New York: Academic Press Inc. 1957.

    Google Scholar 

  • Brown, R. K., R. Delaney, L. Levine andH. V. Vunakis: Studies on the antigenetic structure of ribonuclease. I. General role of hydrogen and disulfide bonds. J. biol. Chem.234, 2043–2049 (1959).

    Google Scholar 

  • Canellakis, E. S.: Metabolism of nucleic acids. Ann. Rev. Biochem.31, 271–300 (1962).

    Google Scholar 

  • Caspersson, T.: Cell growth and cell function. New York: Norton 1950.

    Google Scholar 

  • Cinader, B., andJ. H. Pearce: Immuno-chemical studies on bovine ribonuclease. Symposia on protein structure, p. 541–552. Methuen 1957.

  • Coons, A. H., H. J. Creech, R. N. Jones andE. Berliner: The demonstration of pneumococcal antigen in tissues by the use of fluorescent antibody. J. Immunol.45, 150–170 (1942).

    Google Scholar 

  • Crestfield, A. M., W. H. Stein andS. Moore: On the preparation of bovine pancreatic ribonuclease A. J. biol. Chem.238, 618–621 (1963).

    Google Scholar 

  • Curtain, C. C.: The chromatographic purification of fluorescein-antibody. J. Histochem. Cytochem.9, 484–486 (1961).

    Google Scholar 

  • Daoust, R., andH. Amado: The localization of ribonuclease activity in tissue sections. J. Histochem. Cytochem.8, 131–134 (1960).

    Google Scholar 

  • Ehinger, B., andS. Lagerstedt: Some characteristic reactions catalysed by the ribonuclease activity in sections from fixed tissues. Histochemie1, 331–342 (1959).

    Google Scholar 

  • Hakim, A. A.: Enzymic heterogeneity of crystalline ribonuclease. Arch. Biochem.70, 591–602 (1957a).

    Google Scholar 

  • —— Ribonuclease specificity: Combined action of the major components of crystalline ribonuclease. J. biol. Chem.228, 459–468 (1957b).

    Google Scholar 

  • —— Heterogencous nature of certain ribonucleases. Human urinc and sperm ribonucleases. Arch. Biochem.83, 390–407 (1959).

    Google Scholar 

  • Harris, R. J. C. (Ed.): Protein biosynthesis. New York: Academic Press 1961.

    Google Scholar 

  • Heppel, L. A., andP. R. Whitfeld: Nucleotide exchange reactions catalysed by ribonuclease and spleen phosphodiesterase. I. Synthesis and interconversion of simple esters of ribomononucleotides. Biochem. J.60, 1–7 (1955).

    Google Scholar 

  • —— —— andR. Markham: Nucleotide exchange reactions catalysed by ribonuclease and spleen phosphodiesterase. II. Synthesis of polynucleotides. Biochem. J.60, 8–15 (1955).

    Google Scholar 

  • Hirs, C. H. W., S. Moore andW. H. Stein: A chromatographic investigation of pancreatic ribonuclease. J. biol. Chem.200, 493–506 (1953).

    Google Scholar 

  • Jonsson, N., andS. Lagerstedt: Demonstration of ribonuclease activity in sections from Carnoy-fixed rat pancreas. Experientia (Basel)13, 321–323 (1957).

    Google Scholar 

  • —— The effect of formaldehyde containing fixatives on ribonuclease activity. Histochemie1, 251–256 (1959).

    Google Scholar 

  • Josefsson, L., andS. Lagerstedt: Characterization of ribonuclease and determination of its activity. In: Methods of Biochem. Analysis (D. Glick Ed.), p. 39–74. New York and London: Interscience Publ. 1962.

    Google Scholar 

  • -- A thin-layer chromatographical procedure for determination of ribonuclease activity. (To be published 1963.)

  • Kabat, E. A., andM. M. Mayer: Experimental immunochemistry. Springfield (Ill.): Ch. C. Thomas 1961.

    Google Scholar 

  • Kunitz, M.: Crystalline ribonuclease. J. gen. Physiol.24, 15–32 (1940).

    Google Scholar 

  • Lagerstedt, S.: The release of ribonucleic acid from Carnoy-fixed sections during incubation in McIlvaine's buffer. Experientia (Basel)12, 425–426 (1956).

    Google Scholar 

  • —— Über die Ribonukleaseaktivität in Schnitten von fixierten Geweben. Anat. Anz.105, Erg.-Bd., 244–247 (1959).

    Google Scholar 

  • —— Über die Wirkung der intrazellulären Ribonucleasen. Acta Histochem. (Jena), Suppl.2, 56–69 (1961).

    Google Scholar 

  • Laurell, A. B., andJ. Malmqvist: Separation of the Wassermann reagines by cellulose column chromatography of sera. Acta path. microbiol. scand.51, 187–192 (1961).

    Google Scholar 

  • Lowry, O. H., N. J. Rosenbrough, L. Farr andR. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).

    Google Scholar 

  • Marshall jr., J. M.: Distribution of chymotrypsinogenes, procarboxypeptidase, deoxyribonuclease, and ribonuclease in bovine pancrease. Exp. Cell Res.6, 240–242 (1954).

    Google Scholar 

  • Mayersbach, H. v. u.G. Schubert: Immunohistologische Methoden III. Die unspezifischen Reaktionen zwischen markierten Seren und Geweben bei der immunohistologischen Technik. Acta histochem. (Jena)10, 44–82 (1960).

    Google Scholar 

  • McDonald, M. R., andB. P. Kaufmann: The degradation by ribonuclease of substrates other than ribonucleic acid. J. Histochem. Cytochem.2, 387–393 (1954).

    Google Scholar 

  • Morris, A. J., andS. R. Dickman: Biosynthesis of ribonuclease in mouse pancreas. J. biol. Chem.235, 1404–1408 (1960).

    Google Scholar 

  • Murphy, J. B., andM. W. Kies: Note on spectrophotometric determination of protein in dilute solutions. Biochim. biophys. Acta (Amst.)45, 382–384 (1960).

    Google Scholar 

  • Peterson, E. A., andH. A. Sober: Chromatography of proteins. I. Cellulose ion-exchange adsorbents. J. Amer. chem. Soc.78, 751–755 (1956).

    Google Scholar 

  • Porter, R. R., andE. M. Press: Immunochemistry. Ann. Rev. Biochem.31, 625–652 (1962).

    Google Scholar 

  • Riggs, J. L., R. J. Seiwald, J. H. Burokhalter, C. M. Dawns andT. G. Metcalf: Isothiocyanate compounds as fluorescent labeling agents for immune serum. Amer. J. Path.34, 1081–1092.

  • Singer, S. J., andF. M. Richards: The interaction of subtilisin modified RNase with antisera against native RNase. J. biol. Chem.234, 2911–2914 (1959).

    Google Scholar 

  • Tanford, C., andJ. O. Hauenstein: Identification of the chemical difference between chromatographic components of RNase. Biochim. biophys. Acta (Amst.)19, 535–539 (1956).

    Google Scholar 

  • Waddell, W. J.: A simple ultraviolet spectrophotometric method for the determination of protein. J. Lab. clin. Med.48, 311–314 (1956).

    Google Scholar 

  • Wadsworth, C.: A slide microtechnique for the analysis of immune precipitates in gel. Int. Arch. Allergy10, 355–360 (1957).

    Google Scholar 

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Authors and Affiliations

  1. From the Histological Institute, University of Lund, Sweden

    Berndt Ehinger & Sten Lagerstedt

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  1. Berndt Ehinger
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  2. Sten Lagerstedt
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With 4 Figures in the Text, of which 1 in Colour

Aided by a grant from The Medical Faculty, Lund, to B. E., and by grants from The Swedish Cancer Society, The Julius Andersson and The Otto, Emma and Brita Svensson Foundations to S.L.

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Ehinger, B., Lagerstedt, S. Some histochemically important properties of ribonuclease isolated from fixed tissues. Histochemie 3, 307–314 (1963). https://doi.org/10.1007/BF00736393

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