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Cancer Chemotherapy and Pharmacology

, Volume 32, Issue 5, pp 353–358 | Cite as

Effect of toremifene on the growth, hormone receptors and insulin-like growth factor-1 of hormone-dependent MCF-7 tumors in athymic mice

  • Yuchi Iino
  • Yoshiki Takai
  • Tatsumasa Ando
  • Noritaka Sugamata
  • Michio Maemura
  • Takeshi Takeo
  • Susumu Ohwada
  • Yasuo Morishita
Original Articles Torernitene, MCF-7 tumor, Athymic Mice

Abstract

Toremifene given in different sizes of silastic capsules was used to treat MCF-7 tumors in athmic mice. Toremifene inhibited the estradiol-stimulated growth of MCF-7 tumors in athymic mice. Average serum concentrations of toremifene obtained using a sustained-release preparation of the drug (in 0.5-, 1.0-, and 2.0-cm silastic capsules) increased gradually in a capsule-size-dependent fashion. Much higher levels of toremifene orN-demethyltoremifene were detected in tumors(target tissues of estrogen) as compared with muscles (non-target tissues of estrogen). The concentration of toremifene in serum (i.e., 10–30 ng ml−1) was sufficient to inhibit the estrogen-stimulated growth of MCF-7 tumors at physiological (i.e., 200–400 pg ml−1) serum estradiol concentrations in premenopausal women. No significant difference in estrogen receptor (ER) levels was found between the estradiol-alone group and the toremifene-treated groups. However, the ER levels in the toremifene-alone group and the no-treatment group (no toremifene or estradiol) tended to increase as compared with the estradiol-alone group. Toremifene blocked the estradiol-induced increase in progesterone receptor levels in a dose-dependent fashion. Insulin-like growth factor-1 (IGF-1) levels in the MCF-7 tumors significantly decreased in the toremifene-alone group as compared with the estradiol-alone group. These results show the antiestrogenic action of toremifene on hormone-dependent MCF-7 tumors in athymic mice.

Keywords

Estrogen Receptor Estradiol Serum Estradiol Toremifene Athymic Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Yuchi Iino
    • 1
  • Yoshiki Takai
    • 1
  • Tatsumasa Ando
    • 1
  • Noritaka Sugamata
    • 1
  • Michio Maemura
    • 1
  • Takeshi Takeo
    • 1
  • Susumu Ohwada
    • 1
  • Yasuo Morishita
    • 1
  1. 1.Second Department of SurgeryGunma University School of MedicineMaebashiJapan

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