Virchows Archiv A

, Volume 411, Issue 5, pp 409–414 | Cite as

Effects of estrogens on the testis of transsexuals: A pathological and immunocytochemical study

  • Anna Sapino
  • Alberto Pagani
  • Adriana Godano
  • Gianni Bussolati


Testes from five male-to-female transsexuals removed during sex-reversal surgery after administration of estrogens were studied histologically and with immunoperoxidase procedures to locate estrogen receptors (ER) and related antigens. Atrophy of the seminiferous tubules was observed in all cases; its degree, and a marked decrease in Leydig cells, correlated with low plasma gonadotropin levels (rather than with the duration of treatment). In all cases, the rete testis appeared hyperplastic and the other components (efferent ductules, epididymus) well preserved. The efferent ductules proved to be the only structure where ER can be located by the ER-ICA procedure, whereas staining for the p 29 ER-associated antigen is strongly positive both here and in the rete testis. The immunocytochemical data, in parallel with the observed biological effects, indicate that the rete testis and the efferent ductules and, to a minor degree, the epididymus and Leydig cells are the main direct targets of estrogens in the human testis.

Key words

Estrogens Testis Transsexualism Histology Immunoenzyme techniques 


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  1. Byskov AG (1978) The anatomy and ultrastructure of the rete system in the fetal mouse ovary. Biol Rep 19:720–735Google Scholar
  2. Cano A, Coffer A, Adatia R, Millis R, Rubens R, King RJB (1986) Histochemical studies on oestrogen receptor-related protein in human breast tumors. Cancer Res 46:6475–6480Google Scholar
  3. Carlon N, Stahl A (1973) Les premiers stades dy développement des gonadés chez l'homme et les vertébrés supérieurs. Pathol Biol 21:903–914Google Scholar
  4. Coffer AI, Spiller GM, Lewis KM, King RJB (1985) Immunoradiometric studies with monoclonal antibody against a component related to human estrogen receptor. Cancer Res 45:3694–3698Google Scholar
  5. Depue RH, Pike MC, Henderson BE (1983) Estrogen exposure during gestation and risk of testicular cancer. J Natl Cancer Inst 71:1151–1155Google Scholar
  6. Gooren L (1986) The neuroendocrine response of luteinizinghormone to estrogen administration in heterosexual, homosexual, and transsexual subjects. J Clin Endocrinol Metabol 63:583–588Google Scholar
  7. Hatakeyama S (1965) A study on the interstitial cells of the human testis. Especially on their fine-structural pathology. Acta Pathol Jap 15:155–197Google Scholar
  8. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580Google Scholar
  9. King RJB, Coffer AL, Gilbert J, Lewis K, Nash R, Millis R, Raju S, Taylor RW (1985) Histological studies with a monoclonal antibody raised against a partially purified soluble estradiol receptor preparation from human myometrium. Cancer Res 45:5728–5733Google Scholar
  10. King WJ, Green GL (1984) Monoclonal antibodies localize estrogen receptor in the nuclei of target cells. Nature 307:745–747Google Scholar
  11. Murphy JB, Emmott RC, Hicks LL, Walsh PC (1980) Estrogen receptors in the human prostate, seminal vesicle, epididymis, testis, and genital skin: a marker for estrogen-responsive tissues? J Clin Endocrinol Metabol 50:938–948Google Scholar
  12. Newbold RR, Bullock BC, McLachlan JA (1985) Lesions of the rete testis in mice exposed prenatally to diethylstilbestrol. Cancer Res 45:5145–5150Google Scholar
  13. Oshima H, Sarada T, Ochiai OK, Tamoaki B (1974) Effects of synthetic estrogen upon steroid bioconversation in vitro in testis of patients with prostatic cancer. Invest Urol 12:43–49Google Scholar
  14. Pietribiasi F, Gugliotta P, Racca S, Di Carlo F, Bussolati G (1986) A combined histological, immunocytochemical and biochemical approach in the evaluation of estrogen receptors in breast carcinomas. Int J Biol Markers 1:9–14Google Scholar
  15. Puchtler H, Waldrop ES, Meloan SM, Terry MS, Conner HM (1970) Methacarn (methanol-carnoy) fixation. Histochemie 21:97Google Scholar
  16. Rodriguez-Rigau L, Tcholakian RK, Smith K, Steinberger E (1977) Effect of in vivo estrogen administration on in vitro steroid bioconversation in human testis. In: Troen P, Nankin HR (eds) The testis in normal and infertile men. Raven Press, New York, pp 457–468Google Scholar
  17. Schoen SS, Rush BF Jr (1959) Adenocarcinoma of the rete testis. J Urol 82:356–363Google Scholar
  18. Stumpf WE (1969) Nuclear concentration of3H-estradiol in target tissues. Dry-mount auto radiography of vagina, oviduct, ovary, testis, mammary tumor, liver and adrenal. Endocrinology 85:31–37Google Scholar
  19. Tarakawa N, Huseby RA, Fang SM, Samuels LT (1982) Quantitative changes in estrogen receptor produced by chronic DES treatment of two mouse strains differing in susceptibility to Leydig cell tumor induction. J Steroid Biochem 16:643–652Google Scholar
  20. Yoshitomi K, Morii S (1984) Benign and malignant epithelial tumors of the rete testis in mice. Vet Pathol 21:300–303Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Anna Sapino
    • 1
  • Alberto Pagani
    • 1
  • Adriana Godano
    • 2
  • Gianni Bussolati
    • 1
  1. 1.Department of Biomedical Sciences and Human OncologyUniversity of TurinItaly
  2. 2.Endocrinology ServiceMauriziano HospitalTurinItaly

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