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Cellular and Molecular Neurobiology

, Volume 3, Issue 1, pp 17–26 | Cite as

Inhibition of dopamine-activated adenylate cyclase and dopamine binding by opiate receptors in rat striatum

  • S. Gentleman
  • M. Parenti
  • N. H. Neff
  • C. B. Pert
Article

Summary

  1. 1.

    Low-affinity (micromolar)3H-dopamine binding was measured under conditions which permitted dopamine activation and opiate inhibition of adenylate cyclase in rat striatal membranes. Opiate drugs and peptides inhibited the dopamine binding in the presence of both GTP5 and Gpp(NH)p. Opiate inhibition of adenylate cyclase was, however, observed only in the presence of GTP.

     
  2. 2.

    It is suggested that the dopamine D1 receptor in striatum may be modulated by the opiate delta receptor through a shared guanine nucleotide binding subunit.

     

Key words

dopamine opiates adenylate cyclase striatum 

Abbreviations used

Gpp(NH)p

guanosine-5′-(β-imido)triphosphate

EGTA

ethylene glycol bis(β-aminoethyl ether)

IBMX

isobutyl methylaxanthine

GTP

guanosine 5′-triphosphate

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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • S. Gentleman
    • 1
  • M. Parenti
    • 2
  • N. H. Neff
    • 3
  • C. B. Pert
    • 4
  1. 1.Laboratory of Vision ResearchNational Eye InstituteBethesdaUSA
  2. 2.University of MilanoMilanoItaly
  3. 3.Laboratory of Preclinical PharmacologyNational Institute of Mental HealthWashington, D.C.USA
  4. 4.Section on Brain BiochemistryNeuroscience Branch, National Institute of Mental HealthBethesdaUSA

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