Summary
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1.
Anti-BPM is a neuron-specific antiserum which specifically recognizes the D2 cell adhesion molecule in crossed immunoelectrophoresis of Triton X-100-solubilized brain extracts. Here the effect of this antiserum on thein vitro development of cerebellar neuronal cultures is described.
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2.
The initial adhesion of cells and neurite outgrowth were not influenced by immunoglobulin fractions of anti-BPM. However, after 5 daysin vitro the cultures had become completely disorganized, with the majority of cells being dead at immunoglobulin concentrations greater than 0.5 mg/ml culture medium.
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3.
This effect was seen only with immunoglobulins and their F(ab′)2 fragments, the F(ab′) fragments being without effect. The addition of anti-BPM to 8-day-old cultures resulted in a more rapid and pronounced rate of cell death. In many instances this was preceded by a rapid “destabilization” of culture organization.
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4.
The cytotoxic effect of anti-BPM was neuron specific and the small numbers of astrocytes and fibroblasts found in the cultures were unaffected by prolonged exposure to this serum.
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Regan, C.M., Meier, E. & Balazs, R. The effect of a neuron-specific antiserum, BPM, on thein vitro development of cerebellar granule cells. Cell Mol Neurobiol 7, 61–71 (1987). https://doi.org/10.1007/BF00734990
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DOI: https://doi.org/10.1007/BF00734990