Summary
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1.
The localization of choline acetyltransferase messenger RNA has been studied using a digoxigenin-tailed complementary oligodeoxynucleotide probe forin situ hybridization.
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Putative cholinergic cells of the rat and ferret spinal cord and the ferret retina were labeled.
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This technique affords superior resolution compared to radioactively labeled probes, with apparently equal sensitivity.
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Kennedy, R.E., Hutchins, J.B. Choline acetyltransferase expression studied with an oligonucleotide probe. Cell Mol Neurobiol 12, 309–315 (1992). https://doi.org/10.1007/BF00734931
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DOI: https://doi.org/10.1007/BF00734931