Virchows Archiv A

, Volume 401, Issue 3, pp 289–305 | Cite as

Characterization of B-cell non-Hodgkin's lymphomas

A study using a panel of monoclonal and heterologous antibodies
  • P. van der Valk
  • J. Jansen
  • M. R. Daha
  • C. J. L. M. Meijer


A panel of monoclonal and heterologous antibodies directed against clearly defined antigens was used to characterize the cellular composition of 57 non-Hodgkin's lymphomas, classified according to the Kiel classification, with a slight modification. The antisera were directed against T-lymphocytes and their subsets (Leu1, Leu2a, Leu3a, TA1), B-lymphocytes and their subsets (BA1, BA2, HLA-DR, CR1, sIg), macrophages (TA1, OKM1, anti-human monocyte 1, HLA-DR, CR1), dendritic reticulum cells (CR1, BA2, HLA-DR), interdigitating reticulum cells (HLA-DR, BA1) and Langerhans cells (OKT6, NA1/34). On the basis of the staining pattern of the neoplastic cells with the antibodies used and the nature and number of admixed cells, in particular T-cell subsets, dendritic reticulum cells and macrophages, the NHL could be divided into groups which correspond to the different diagnostic categories of the Kiel classification. Furthermore, the results underlined the existence of intermediate lymphocytic lymphoma as a separate diagnostic category. Histogenetically, the marker pattern of the neoplastic cells and the number and arrangement of the admixed cells are consistent with the view that at least two different lines of B-cell lymphomas can be recognized. One is related to the germinal centre cell reaction (to which B-lymphoblastic (Burkitt type), centroblastic, centroblastic/centrocytic, centrocytic, and intermediate lymphocytic lymphoma, and polymorphic immunocytoma belong) and the other is related to the plasma cell reaction (including chronic lymphocytic leucaemia and lymphoplasmacytoid immunocytoma), whereas B-immunoblastic lymphoma can originate from either line. Thus, polymorphic immunocytoma is a follicle centre cell lymphoma with differentiation into plasma cells rather than a lymphoplasmacytoid immunocytoma with blastic cells.

Key words

B-cell Non-Hodgkin's lymphomas Monoclonal anti-bodies Dendritic reticulum cells 


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  1. Abramson CS, Kersey JH, LeBien TW (1981) A monoclonal antibody (BA1) reactive with cells of the human B lymphocyte lineage. J Immunol 126:83–88Google Scholar
  2. Breard J, Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124:1943–1948Google Scholar
  3. Daha MR, Kok DJ, Van Es LA (1982) Regulation of the C3 nephritic factor stabilized C3/C5 convertase of complement by purified human erythrocyte C3b receptor. Clin Exp Immunol 50:209–214Google Scholar
  4. Dvoretsky P, Wood GS, Levy R, Warnke RA (1982) T lymphocyte subsets in follicular lymphomas compared with those in non-neoplastic lymph nodes and tonsils. Human Pathol 13:618–625Google Scholar
  5. Evans RL, Wall SW, Platsoucas CO, Siegal FP, Fikrig SM, Testa CM, Good RA (1981) Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci USA 78:544–548Google Scholar
  6. Fearon DT (1979) Regulation of the amplification C3 convertase of human complement by an inhibitory protein isolated from human erythrocyte membrane. Proc Natl Acad Sci USA 76:5867–5871Google Scholar
  7. Foon KA, Schroff RW, Gale RP (1982) Surface markers on leukemia and lymphoma cells: Recent advances. Blood 60:1–19Google Scholar
  8. Gajl-Peczalska KJ, Bloomfield CD, Frizzera G, Kersey JH, LeBien TW (1982) Diversity of phenotypes of non-Hodgkin's malignant lymphomas. From: B- and T-cell Tumours. UCLA Symposia on Molecular and Cellular Biology Vol 24. Eds. E Vittetta, CF Fox. Acad Press New York, pp 63–67Google Scholar
  9. Janossy G, Thomas JA, Pizzolo G, Granger SM, McLaughlin J, Habeshaw JA, Stansfeld AG, Sloane J (1980). Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies. Br J Cancer 42:224–242Google Scholar
  10. Kersey JH, LeBien TW, Abramson CS, Newman R, Sutherland R, Greaves M (1981) p 24. A human leukemia associated and lymphopoietic progenitor cell surface structure identified with monoclonal antibody. J Exp Med 153:726–731Google Scholar
  11. LeBien TW, Kersey JH (1980) A monoclonal antibody (TA1) reactive with human T-lymphocytes and monocytes. J Immunol 125:2208–2214Google Scholar
  12. Ledbetter JA, Evans RL, Lipinski M, Rundles C, Herzenberg LA (1981) Evolution conservation of surface molecules that distinguish T-lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med 153:310–323Google Scholar
  13. Lennert K (1978) Malignant lymphomas, other than Hodgkin's disease. Handbuch der speziellen pathologischen Anatomie und Histologie I/3/B Springer, Berlin Heidelberg New YorkGoogle Scholar
  14. Loo EM van der, Valk P van der, Jansen J, Daha MR, Meijer CJLM (1983) Distribution of lymphoid and dendritic cells in lymphatic tissue. Analysis by monoclonal antibodies. Virchows Arch [Cell Pathol]Google Scholar
  15. Lukes RJ, Collins RD (1975) New approaches to the classification of the lymphomata. Br J Cancer 31 suppl II 1–28Google Scholar
  16. Mann RB, Jaffe ES, Braylon RC, Nanba K, Frank MM, Ziegler JL, Berard CW (1976) Non-endemic Burkitt's lymphoma A B-cell tumor related to the germinal centers. N Engl J Med 295:685–691Google Scholar
  17. Mann RB, Jaffe ES, Berard CW (1979) Malignant lymphomas - A conceptual understanding of morphologic diversity. Am J Pathol 94:104–191Google Scholar
  18. McMichael AJ, Pilch JR, Galfre G, Mason DY, Fabre JW, Milstein C (1979) A human thymocyte antigen defined by hybrid myeloma monoclonal antibody. Eur J Immunol 9:205–210Google Scholar
  19. Murphy GF, Ban AR, Sato S, Mihm jr MC, Harrist TJ (1981) A new immunologic marker for human Langerhans cells. N Engl J Med 304:791–792Google Scholar
  20. Nanba K, Jaffe ES, Braylan RC, Soban EJ, Berard CW (1977) Alkaline phosphatase positive malignant lymphomas. A subtype of B-cell lymphomas. Am J Clin Pathol 68:535–542Google Scholar
  21. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF (1980) Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-lineage. Proc Natl Acad Sci USA 77:1588–1592Google Scholar
  22. Stein H (1978) The immunologic and immunochemical basis for the Kiel classification. In: Lenner K Malignant lymphomas, other than Hodgkin's disease. Springer, Berlin Heidelberg New YorkGoogle Scholar
  23. Stein H, Tolksdorf G (1980) Development and differentiation of the T-cell and B-cell systems: a perspective. In: van den Tweel JG, Taylor C, Bosman FT (eds) Malignant lymphoproliferative diseases. Leiden University Press, The Hague/Boston/New York, pp 13–29Google Scholar
  24. Stein H, Mason DY, Gerdes J, Ziegler A, Naiem M, Wernet P, Lennert K (1981) Immunohistology of B-cell lymphomas. In: Knapp W (ed) Leukemia markers, Academic Press, London, pp 99–108Google Scholar
  25. Stein H, Staudinger M, Tolksdorf G, Lennert K (1981) Immunologic markers in the differential diagnosis of non-Hodgkin's Lymphomas. J Cancer Res Clin Oncol 101:29–42Google Scholar
  26. Ugolini V, Nunez G, Smith GR, Stastny P, Capra JD (1980) Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci USA 77:6764–6768Google Scholar
  27. Valk P van der, Velde J te, Jansen J, Ruiter DJ, Spaander PJ, Cornelisse CJ, Meijer CJLM (1981) Malignant lymphoma of true histiocytic origin: Histiocytic sarcoma. A morphological, ultrastructural, immunological, cytochemical and clinical study of 10 cases. Virchows Arch [Pathol Anat] 391:249–265Google Scholar
  28. Valk P van der, Hermans J, Brand R, Cornelisse CJ, Spaander PJ, Meijer CJLM (1982) Morphometric characterization of diffuse large-cell (Histiocytic) lymphomas. Am J Pathol 107:327–335Google Scholar
  29. Valk P van der, Mosch A, Kurver PHJ, Meijer CJLM (1983) Morphometric characterization of 52 B-cell Non-Hodgkin's lymphomas. J Clin Pathol 36:289–297Google Scholar
  30. Veldman JE (1970) Histophysiology and electron microscopy of the Immune Ressponse. Thesis Groningen. N.V. Boekdrukkerij Dijkstra NiemeyerGoogle Scholar
  31. Wakefield JD, Thorbecke GJ (1968) Relationship of germinal centers in lymphoid tissue to immunological memory. J Exp Med 128:153–187Google Scholar
  32. Wang CY, Good RA, Ammirati P, Dymbort G, Evans RL (1980) Identification of p 69, 71 complex expressed on human T-cells sharing determinants with B type chronic lymphocytic leukemic cells. J Exp Med 151:1539–1544Google Scholar
  33. Warnke R, Levy R (1980) Detection of T- and B-cell antigens with hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem 28:771–776Google Scholar
  34. Warnke R, Miller R, Grogan R, Pederson M, Dilley J, Levy R (1980) Immunological phenotype in 30 patients with diffuse large-cell lymphoma. N Engl J Med 303:293–300Google Scholar
  35. Weisenburger DD, Nathwani BN, Diamond LW, Winberg CD, Rappaport H (1981) Malignant lymphoma, intermediate lymphocytic type. A clinicopathologic study of 42 cases. Cancer 48:1415–1425Google Scholar
  36. Weisenburger DD, Kim H, Rappaport H (1982) Mantle-zone lymphoma. A follicular variant of intermediate lymphocytic lymphoma. Cancer 49:1429–1438Google Scholar
  37. Wright DH (1982) The identification and classification of non-Hodgkin's lymphomas: a review. Diagnostic Histopathology 5:73–11Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • P. van der Valk
    • 1
  • J. Jansen
    • 2
  • M. R. Daha
    • 3
  • C. J. L. M. Meijer
    • 4
    • 5
  1. 1.Departments of PathologyUniversity Medical Centre, LeidenAmsterdamThe Netherlands
  2. 2.HaematologyFree University AmsterdamAmsterdamThe Netherlands
  3. 3.NephrologyFree University AmsterdamAmsterdamThe Netherlands
  4. 4.Stichting Samenwerking Delftse Ziekenhuizen, Department of Pathology DelftFree University AmsterdamAmsterdamThe Netherlands
  5. 5.Department of PathologyFree UniversityAmsterdamThe Netherlands

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