Advertisement

Virchows Archiv A

, Volume 402, Issue 4, pp 425–438 | Cite as

Calcium redistribution, calcification and stone formation in the parotid gland during experimental stimulation and hypercalcaemia

Cytochemical and X-ray microanalytical investigations
  • M. Westhofen
  • H. Schäfer
  • G. Seifert
Article

Summary

Distribution and redistribution of intra- and pericellular calcium was investigated in the parotid gland of rats under secretory stimulation and hypercalcaemia. The effects of hypercalcaemia and secretory stimulation and of the combination of both were compared. Calcium content was determined by atomic absorption spectrometry. Calcium distribution within the tissue was demonstrated by light microscopical (GBHA) staining and electron microscopical (pyroantimonate method) cytochemistry in combination with X-ray microanalysis. Typical calcium depot sites were the basal and cellular membranes, the calcium buffer organelles (i.e. mitochondria) the secretory granules and the acinar lumina. After stimulation (by isoprenalin) a decrease of calcium-enriched secretory granules and a depletion of intracellular calcium buffer organelles occurred. During hypercalcaemia (induced by dihydrotachysterol), a calcium overloading of the cell membrane and intracellular buffer organelles without calcification was observed. Combined stimulation and hypercalcaemia induced an excessive calcium overloading of all intra-and extracellular calcium depots with excessive calcium release into the acinar lumina resulting in calcium phosphate aggregates and stone formation. Secretory stimulation and simultaneous hypercalcaemia exert potentiating effects on intracellular and intraluminal calcification proposing an importance for pathogenesis of human sialolithiasis.

Key words

Calcium redistribution Calcification Parotid gland Cytochemistry X-ray microanalysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. 1.
    Amsterdam A, Ohad I, Schramm M (1969) Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle. J Cell Biol 41:753–773Google Scholar
  2. 2.
    Boskey AL, Boyan-Sayers BD, Mandel ID (1981) Lipids associated with mineralisation of human submandibulary gland sialoliths. Arch Oral Biol 26:779–785Google Scholar
  3. 3.
    Carafoli E, Crompton M (1978) The regulation of intracellular calcium by mitochondria. In: Calcium transport and cell function. Ann Acad Sci 307:269–284Google Scholar
  4. 4.
    Chilla R, Witzemann M, Opaitz M, Arglebe C (1981), Possible involvement of parotid beta-adrenergic receptors in the etiology of sialadenosis. Arch Otorhinolaryngol 230:113–120Google Scholar
  5. 5.
    Donath K, Spillner M, Seifert G (1974) The influence of the autonomic nervous system on the ultrastructure of the parotid acinar cells. Virchows Arch [Pathol Anat] 364:15–33Google Scholar
  6. 6.
    Donath K, Seifert G (1975) Ultrastructural studies of parotid glands in sialadenosis. Virchows Arch [Pathol Anat] 365:119–135Google Scholar
  7. 7.
    Douglas WW, Rubin RP (1961) The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol 159:40–57Google Scholar
  8. 8.
    Douglas WW, Poisner AM (1963) The influence of calcium on the secretory response of the submaxillary gland to acetylcholine or to noradrenaline. J Physiol 165:528–541Google Scholar
  9. 9.
    Douglas WW (1968) Stimulus secretion coupling: the concept and clues from chromaffin and other cells. Br J Pharmacol 34:648–658Google Scholar
  10. 10.
    Dreisbach RH (1964) Effect of isoproterenol on calcium metabolism in rat salivary gland. Proc Soc Exp Biol Med 116:935–960Google Scholar
  11. 11.
    Ginsborg BL, House CR, Mitchel MR (1980) A calcium readmission response from Nauphoeta salivary gland acinar cells. J Physiol 304:437–447Google Scholar
  12. 12.
    Immenkamp M, Seifert G (1969) Zur Pathogenese der experimentellen Speicheldrüsen-Calciphylaxie. Virchows Arch [Pathol Anat] 347:211–224Google Scholar
  13. 13.
    Martin K, Burgen ASV (1962) Changes in permeability of the salivary gland caused by sympathetic stimulation and by catecholamines. J Gen Physiol 46:225–243Google Scholar
  14. 14.
    Perkin Elmer (1972) Analytische Methoden der Atom-AbsorptionsspektrophotometrieGoogle Scholar
  15. 15.
    Peterfy C, Tenenhouse A (1982) Vitamin D receptors in isolated rat parotid gland acinar cells. Biochemica et Biophysica Acta 721:158–163Google Scholar
  16. 16.
    Schäfer H, Klöppel G (1974a) Demonstration of calcium in pancreatic islets. Light microscopic observations in activated and unactivated B-cells of mice. Virchows Arch [Pathol Anat] 362:1–12Google Scholar
  17. 17.
    Schäfer H, Klöppel G (1974b) The significance of calcium in insulin secretion. Ultrastrucural studies on identification and localisation of calcium in activated and unactivated B-cells of mice. Virchows Arch [Pathol Anat] 362:231–245Google Scholar
  18. 18.
    Schäfer H (1979) Zellcalcium und Zellfunktion. G. Fischer Verlag, StuttgartGoogle Scholar
  19. 19.
    Seifert G (1962a) Experimentelle Speicheldrüsenvergrößerung nach Einwirkung von Noradrenalin. Beitr Pathol. Anat 126:321–351Google Scholar
  20. 20.
    Seifert G (1962b) Elektronenmikroskopische Befunde an Speicheldrüsenacini nach Einwirkung von Noradrenalin. Beitr Pathol Anat 127:112–136Google Scholar
  21. 21.
    Selye H, Cantin M, Veilleux R (1961) Abnormal growth and sclerosis of the salivary glands induced by chronic treatment with isoproterenol. Growth 25:243Google Scholar
  22. 22.
    Sharer WG, Hine MK, Levy BM (1974) Physical and chemical injuries of the oral cavity. In: A textbook of oral pathology 3rd edn. WB. Saunders Company Philadelphia, London, Toronto p 513Google Scholar
  23. 23.
    Simson JAV (1972) Evidence of cell damage in rat salivary glands after isoproterenol. Anat Rec 173:437–441Google Scholar
  24. 24.
    Simson JAV, Spicer SS (1974) Cytochemical evidence for cation fluxes in parotid acinar cells following stimulation by isoproterenol. Anat Rec 178:145–167Google Scholar
  25. 25.
    Simson JAV (1975) Selective localisation of cations with variants of the potassium(pyro)antimonate technique. J Histochem cytochem 23:575–598Google Scholar
  26. 26.
    Wah Leung S, Draus FJ (1962) The calcium binding characteristics of a salivary gland mucoid. Arch Oral Biol 7:327–332Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • M. Westhofen
    • 1
  • H. Schäfer
    • 2
  • G. Seifert
    • 2
  1. 1.HNO-Klinik der Universitätsklinik Hamburg-EppendorfHamburg 20
  2. 2.Institut für Pathologie der Universitätsklinik Hamburg-EppendorfHamburg 20Federal Republic of Germany

Personalised recommendations