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Virchows Archiv A

, Volume 411, Issue 1, pp 79–85 | Cite as

Quantitation of anionic sites in glomerular capillar basement membranes of Samoyed dogs with hereditary glomerulopathy

  • Paul S. Thorner
  • Barbara Jansen
  • Jon Liang
  • Reuben Baumal
  • Victor Valli
Article

Summary

Samoyed hereditary glomerulopathy (SHG) is an X-linked dominant disease characterized by proteinuria and renal failure in affected male dogs. Electron microscopic examination of glomerular capillary basement membranes (GCBM) shows widespread multilaminar splitting of the lamina densa, identical to that in Alport's syndrome. Anionic sites in GCBM of three affected males and five unaffected dogs were labeled using polyethyleneimine to determine whether proteinuria was associated with an alteration in their number. No significant differences were noted in the number of anionic sites in the lamina rara externa, whereas small but statistically significant increases were seen in the number of sites in the lamina rara interna of affected males. In the lamina densa, affected males showed a striking increase in anionic sites, particularly in regions of GCBM which were split. Thus, although proteinuria in some glomerular diseases has been attributed to a reduction in anionic sites in GCBM, this was not so in SHG.

Key words

Anionic sites Heparan sulfate proteoglycans 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Paul S. Thorner
    • 1
    • 2
  • Barbara Jansen
    • 3
  • Jon Liang
    • 1
  • Reuben Baumal
    • 1
    • 2
  • Victor Valli
    • 3
  1. 1.Departments of PathologyThe Hospital for Sick Children 555 University Avenue TorontoOntarioCanada
  2. 2.University of TorontoToronto
  3. 3.Ontario Veterinary CollegeGuelphCanada

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