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Cellular and Molecular Neurobiology

, Volume 10, Issue 1, pp 33–39 | Cite as

Localization of nerve growth factor receptor mRNA in the rat basal forebrain within situ hybridization histochemistry

  • J. E. Springer
  • E. Robbins
  • S. Meyer
  • F. BaldinoJr.
  • M. E. Lewis
Article

Summary

  1. 1.

    In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain.

     
  2. 2.

    In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis.

     
  3. 3.

    This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques.

     
  4. 4.

    No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe.

     
  5. 5.

    We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.

     

Key words

in situ hybridization nerve growth factor (NGF) NGF receptor (NGFR) cholinergic neurons 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • J. E. Springer
    • 1
  • E. Robbins
    • 2
  • S. Meyer
    • 1
  • F. BaldinoJr.
    • 2
  • M. E. Lewis
    • 2
  1. 1.Department of Neurology, Hahnemann Institute of NeuroscienceHahnemann University School of MedicinePhiladelphiaUSA
  2. 2.Cephalon, Inc.West ChesterUSA

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