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Glycoconjugate Journal

, Volume 8, Issue 1, pp 29–37 | Cite as

Structures of the α(1-3)-galactose-containing asparagine-linked glycans of a Lewis lung carcinoma cell subline resistant toAleuria aurantia agglutinin: elucidation by1H-NMR spectroscopy

  • Henri Debray
  • Danuta Dus
  • Jean-Michel Wieruszeski
  • Gérard Strecker
  • Jean Montreuil
Papers

Abstract

Four bi-antennary glycan fractions of theN-acetyllactosamine-type, derived from a Lewis lung carcinoma (LL2) cell subline resistant to theAleuria aurantia agglutinin were studied by 400 MHz1H-NMR spectroscopy. By this method, their antennae were found to be terminated either by α(2-3 or 6)-linkedN-acetylneuraminic acid or α(1-3)-linked galactose residues. The primary structure of glycans of these four glycopeptide or derived oligosaccharide-alditols has been determined in full detail.

Keywords

1H-NMR asparagine-linked glycans lectin resistance 

Abbreviations

NAc

N-acetyl group

NGc

N-glycolyl group

GlcNAc

N-acetylglucosamine

NeuAc

N-acetylneuraminic acid

NeuGc

N-glycolylneuraminic acid

Man

mannose

Gal

galactose

Fuc

fucose

Con A

concanavalin A

LCA

Lens culinaris agglutinin

AAA

Aleuria aurantia agglutinin

WGA

Wheat germ agglutinin

RCA II

Ricinus communis agglutinin II

PBS

phosphate buffered saline, 0.01m Na2HPO4/0.14m NaCl, pH 7.2

HPLC

high performance liquid chromatography

EMEM

Eagle's Minimal Essential Medium

LecR

lectin resistant

MG

α-methylglycoside

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Copyright information

© Chapman and Hall Ltd 1991

Authors and Affiliations

  • Henri Debray
    • 1
  • Danuta Dus
    • 2
  • Jean-Michel Wieruszeski
    • 1
  • Gérard Strecker
    • 1
  • Jean Montreuil
    • 1
  1. 1.Laboratoire de Chimie Biologique, Unité Mixte du CNRS No 111Université des Sciences et Techniques de Lille Flandres-ArtoisVilleneuve d'Ascq CedexFrance
  2. 2.Department of Tumor Immunology, Hirszfeld Institute of Immunology and Experimental TherapyPolish Academy of SciencesWroclawPoland

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