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Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a−b+) p phenotype: absence of blood group structures based on the globoseries

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Abstract

Total neutral glycolipid fractions were isolated from kidney and ureter tissue obtained at autopsy of an individual of the rare blood group A1 Le(a−b+) p. The amount of glycolipids isolated were 3.7 and 2.5 mg g−1 dry tissue weight for the kidney and ureter tissue, which is in the range of reference blood group P kidneys. Part of the kidney glycolipid fraction was subfractionated by HPLC. Glycolipid compounds were structurally characterized by thin-layer chromatography (chemical detection and immunostaining with monoclonal antibodies), proton NMR spectroscopy and mass spectrometry. Globotriaosyl- and globotetraosyl-ceramides, which are the major compounds in kidneys of P individuals, were absent in the p kidney, and a comparatively increased amount of monoglycosyland lactosylceramides was found. A shift to longer fatty acyl chains in the ceramide part of lactosylceramides was noted. Elongated globoseries compounds with five to seven sugar residues, including the blood group A type 4 chain structure, were lacking. A slight increase in neolactotetraosyl- and blood group X pentaglycosyl-ceramides was noticed. The study confirms an enzymatic block in the conversion of lactosylceramide to elongated globoseries compounds in the kidney tissue similar to that of erythrocytes of p individuals.

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Authors and Affiliations

  1. Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska Hospital, 413 45, Göteborg, Sweden

    Karin Lindström, Sohbat Ghardashkani & Bo E. Samuelsson

  2. Department of Medical Biochemistry, University of Göteborg, 413 90, Göteborg, Sweden

    Per-Åke Jovall

  3. Department of Surgery, University of Göteborg, Sahlgrenska Hospital, 413 45, Göteborg, Sweden

    Michael E. Breimer

Authors
  1. Karin Lindström
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  2. Per-Åke Jovall
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  3. Sohbat Ghardashkani
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  4. Bo E. Samuelsson
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  5. Michael E. Breimer
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Additional information

Abbreviations: for blood group glycolipid antigens the short hand designation stands for: blood group — number of sugar residues — type of carbohydrate chain. Thus A-7-4 means a blood group A heptaglycoconjugate on a type 4 chain. The sugar types are abbreviated for mass spectrometry to Hex for hexose, HexNAc forN-acetylhexosamine and dHex for deoxyhexose. HPLC, high-performance liquid chromatography; HPTLC, high performance thin layer chromatography; EI, electron impact ionisation; LSI, liquid secondary ion; MS, mass spectrometry; NMR, nuclear magnetic resonance.

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Lindström, K., Jovall, PÅ., Ghardashkani, S. et al. Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a−b+) p phenotype: absence of blood group structures based on the globoseries. Glycoconjugate J 13, 307–313 (1996). https://doi.org/10.1007/BF00731505

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  • DOI: https://doi.org/10.1007/BF00731505

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