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Sulfatide prolongs blood-coagulation time and bleeding time by forming a complex with fibrinogen

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Abstract

Sulfatides (galactosylceramide I3-sulfate), which are found in serum lipoproteins of various mammals, effectively increased prothrombin time (anticoagulant effect) and also effectively prolonged bleeding time (anti-platelet effect). When equal volumes of a homogeneous micellar solution of sulfatide and fibrinogen in phosphate-buffered saline were mixed, an insoluble complex precipitated. Analysis of the precipitated complex showed that the molar ratio of sulfatide to fibrinogen was about 400:1. These results indicate that the sulfatide micelle binds tightly to fibrinogen and thereby interferes with both fibrin gel formation (anticoagulant activity) and platelet function.

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Abbreviations

BSA:

bovine serum albumin

PBS:

phosphate-buffered saline

PT:

prothrombin time

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Authors and Affiliations

  1. Research Center on Aging and Adaptation Medicine, Shinshu University School of Medicine, 390, Matsumoto, Japan

    Atsushi Hara, Kei-Ichi Uemura & Tamotsu Taketomi

Authors
  1. Atsushi Hara
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  2. Kei-Ichi Uemura
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  3. Tamotsu Taketomi
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Dedicated to Dr Sen-itiroh Hakomori in celebration of his 65th birthday.

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Hara, A., Uemura, KI. & Taketomi, T. Sulfatide prolongs blood-coagulation time and bleeding time by forming a complex with fibrinogen. Glycoconjugate J 13, 187–194 (1996). https://doi.org/10.1007/BF00731493

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  • DOI: https://doi.org/10.1007/BF00731493

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