Abstract
In the present study, three extremely minor but novel Chol-1 antigens, termed X1, X2, and X3 have been isolated from bovine brain gangliosides. Based on the results of sialidase degradation, TLC-immunostaining with anti-Chol-1 antibody and fast atom bombardment mass spectrometry, their chemical structures were identified as:
The yields of GM1α, GD1aα, and GT1bα, were approximately 150, 20, and 10 µg, respectively, from 10 g of the bovine brain ganglioside mixture. In conjunction with our previous observations, all gangliosides with anti-Chol-1 reactivity were found to contain a common sialyl α2–6N-acetylgalactosamine residue, indicating that this unique sialyl linkage is the specific antigenic determinant. We subsequently examined the biosyntheses of the three novel Chol-1 gangliosides using rat liver Golgi fraction as an enzyme source. The results showed that GM1α, GD1aα, and GT1bα were synthesized from asialo-GM1, GM1a, and GD1b, respectively, by the action of a GalNAc α2-6sialyltransferase.
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Abbreviations: The nomenclature used for gangliosides is based on the system of Svennerholm [37] and Hirabayashiet al. [8, 12]. Cer, ceramide; Gal, galactose; Glc, glucose; GalNAc,N-acetylgalactosamine; NeuAc,N-acetylneuraminic acid; NeuGc,N-glycolylneuraminic acid; Hex, hexose; HexNAc,N-acetylhexosamine; TLC, thin layer chromatography; HPTLC, high performance thin layer chromatography; ELISA, enzyme-linked immunosorbent assay; FAB-MS, fast atom bombardment-mass spectrometry; HD, Hanganutziu-Deicher.
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Irie, F., Kurono, S., Li, YT. et al. Isolation of three novel cholinergic neuron-specific gangliosides from bovine brain and theirin vitro syntheses. Glycoconjugate J 13, 177–186 (1996). https://doi.org/10.1007/BF00731492
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DOI: https://doi.org/10.1007/BF00731492