Abstract
In this study, the antibody 3G9-A was assayed for activity against human erythrocyte glycosphingolipids. The antibody was found to recognize glycosphingolipid components from blood group A erythrocytes but not glycosphingolipids from blood group B or O erythrocytes. Subsequent investigation revealed that the glycosphingolipid components recognized by the antibody were also recognized by a blood group A specific monoclonal antibody. The structures of two of the isolated active glycosphingolipid components were structurally characterized using proteon nuclear magnetic resonance (1H NMR) and gas chromatography-mass spectrometry (GC-MS) techniques and were found to consist of two blood group A glycosphingolipids; the type 2 chain Ab and type 3 chain Aa glycosphingolipids. Subsequent analysis of the remaining active components by GC-MS and immunostaining techniques revealed that all of the active components were blood group A glycosphingolipids. Furthermore, structural studies of the active components suggested that the epitope of the antibody consisted of the group A trisaccharide, GalNAcα1,3(Fucα1,2)Gal.
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Abbreviations
- GC-MS:
-
gas chromatography-mass spectrometry
- 1H NMR:
-
proton nuclear magnetic resonance
- Gal:
-
d-galactose
- Glc:
-
d-glucose
- Fuc:
-
l-fucose
- GalNAc:
-
N-acetylgalactosamine
- GlcNAc:
-
N-acetylglucosamine
- Cer:
-
ceramide
- mAb:
-
monoclonal antibody
- BSA:
-
bovine serum albumin
- PBS:
-
phosphate buffered saline
- FID:
-
free induction decay
- PMAA:
-
partially methylated alditol acetates
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Briggs, J.B., Mandrell, R. & Macher, B.A. Structural characterization of blood group A glycosphingolipids recognized by the antibody 3G9-A. Glycoconjugate J 13, 487–494 (1996). https://doi.org/10.1007/BF00731482
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DOI: https://doi.org/10.1007/BF00731482